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1.5.1.6: formyltetrahydrofolate dehydrogenase

This is an abbreviated version!
For detailed information about formyltetrahydrofolate dehydrogenase, go to the full flat file.

Word Map on EC 1.5.1.6

Reaction

10-formyltetrahydrofolate
+
NADP+
 +
H2O
=
tetrahydrofolate
 +
CO2
 +
NADPH
 +
H+

Synonyms

10-FDH, 10-formyl tetrahydrofolate:NADP oxidoreductase, 10-formyl-H2PtGlu:NADP oxidoreductase, 10-formyl-H4folate dehydrogenase, 10-formyltetrahydrofolate dehydrogenase, 10-fTHF dehydrogenase, 10-FTHFDH, ALDH1L1, ALDH1L2, dehydrogenase, formyltetrahydrofolate, FBP-CI, FBP-CI proteins, FDH, folate-binding proteins, cytosol I, More, mtFDH, N10-formyltetrahydrofolate dehydrogenase, proteins, folate-binding, cytosol I

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.6 formyltetrahydrofolate dehydrogenase

Engineering

Engineering on EC 1.5.1.6 - formyltetrahydrofolate dehydrogenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C707A
-
catalytically inactive mutant
S374A
-
the mutant is activated by 4'-phosphopantetheinyl transferase
S374A/S375A
-
the mutant is not activated by 4'-phosphopantetheinyl transferase
S375A
-
the mutant is not activated by 4'-phosphopantetheinyl transferase
C152A
95% of the activity with wild-type enzyme
C17A
94% of the activity with wild-type enzyme
C191A
96% of the activity with wild-type enzyme
C238A
102% of the activity with wild-type enzyme
C707A
C86A
110% of the activity with wild-type enzyme
D142A
-
mutant with a complete loss of 10-formyltetrahydrofolate dehydrogenase and hydrolase activity, aldehyde dehydrogenase activity is similar to wild-type FDH
D399A
90% of the dehydrogenase activity of the wild-type enzyme
E398A
90% of the dehydrogenase activity of the wild-type enzyme
F384_V405del
no dehydrogenase activity
G397A
as active as wild-type enzyme
H106A
-
insoluble recombinant protein
H106K
-
mutant with a complete loss of 10-formyltetrahydrofolate dehydrogenase and hydrolase activity, aldehyde dehydrogenase activity is similar to wild-type FDH
K394A
75% of the dehydrogenase activity of the wild-type enzyme
K394A/L395A/R396A/G397A/E398A/D399A
no dehydrogenase activity
K394_D399del
no dehydrogenase activity
L395A
80% of the dehydrogenase activity of the wild-type enzyme
R396A
as active as wild-type enzyme
S354A
-
no dehydrogenase activity
additional information
-
deletion mutation in the 10-formyltetrahydrofolate structural gene in homozygous mice with absence of FDH mRNA and enzyme activity, total folate pool is decreased and the level of tetrahydrofolate is markedly depleted, pairs of homozygotes have a dramatically decreased reproductive efficiency, may be attributable to fetal lethality