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1.5.3.16: spermine oxidase

This is an abbreviated version!
For detailed information about spermine oxidase, go to the full flat file.

Word Map on EC 1.5.3.16

Reaction

spermine
+
O2
 +
H2O
=
spermidine
 +
3-aminopropanal
 +
H2O2

Synonyms

AtPAO1, AtPAO4, AtPAO5, EC 1.5.3.11, Fms1 protein, GhPAO, hSMO, MmSMO, mSMO, mSMOalpha, mSMOmu, PAO, PAO1, PAO4, PAO5, PAO6, PAO7, PAOh1, PAOh1/SMO, SelPAO5, SMO, SMO(PAOh1), SMO/PAOh1, SMO5, SMOX, spermine oxidase, Spm oxidase, thermospermine oxidase

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.3 With oxygen as acceptor
                1.5.3.16 spermine oxidase

Cloned

Cloned on EC 1.5.3.16 - spermine oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AtPAO1 cDNA is isolated and cloned in a vector for heterologous expression in Escherichia coli
AtPAO1, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli, expression of GFP-tagged AtPAO3 in Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method
-
cDNA is transiently transfected into HEK-293 cells
constitutive expression of GhPAO in Arabidopsis thaliana produces improved resistance to Verticillium dahliae (a destructive, soil-borne fungal pathogen) and maintains putrescine, spermidine and spermine at high levels
-
expressed in Escherichia coli BL21 DE3
expressed in HEK 293 cells
expression in Escherichia coli
expression in Escherichia coli as a His-tagged protein
expression in Escherichia coli BL21 DE3
expression in HEK-293 cells
-
expression of His-tagged AtPAO4 in Escherichia coli strain BL21(DE3), transient expression of isozyme AtPAO4 in Arabidopsis thaliana root cell peroxisomes as monomeric red fluorescent protein fusion protein
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
expression of mutant mSMOmuDELTA (with a deletion of the nuclear domain) in Escherichia coli
FMS1 gene cloned in frame with a C-terminal 6x His tag and expressed as a recombinant protein in Escherichia coli
-
gene AtPAO1, construction of AtPAO::GUS transgenic Arabidopsis thaliana plants
-
gene hSMO, expression in Escherichia coli strain BL21(DE3)
-
gene SMOX, phylogenetic analysis
isozymes SMOalpha and SMOmu, exon structures of murine SMO splice variants, overview
-
mSMO splice variants, expression in Escherichia coli and in in murine neuroblastoma N18TG2 cells
production of recombinant SelPAO5 protein in Escherichia coli
SMO/PAOh1 cDNA is subcloned into the pET15b bacterial expression vector, expression in Escherichia coli. NCI-H157 human non-small cell lung carcinoma cells are stably transfected, and individual clones selected that overexpress the enzyme
SMO5 cDNA is subcloned into the pET15b bacterial expression vector, expression in Escherichia coli. NCI-H157 human non-small cell lung carcinoma cells are stably transfected, and individual clones selected that overexpress the enzyme
subcloned and expressed in secreted and secreted-tagged forms into Escherichia coli BL21 DE3 cells