1.5.8.4: dimethylglycine dehydrogenase
This is an abbreviated version!
For detailed information about dimethylglycine dehydrogenase, go to the full flat file.
Word Map on EC 1.5.8.4
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1.5.8.4
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sarcosine
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fad
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flavinylated
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folate-binding
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wagner
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medicine
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diagnostics
- 1.5.8.4
- sarcosine
- fad
-
flavinylated
-
folate-binding
-
wagner
- medicine
- diagnostics
Reaction
Synonyms
Csal_0990, DdhC, DMGDH, EC 1.5.99.2, Me2GlyDH, mMe2GlyDH, N,N-dimethylglycine oxidase, pMe2GlyDH
ECTree
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Cloned
Cloned on EC 1.5.8.4 - dimethylglycine dehydrogenase
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development of an intracellular, heterologous expression system in Komagataella phaffii (formerly known as Pichia pastoris). Initial attempts to express the gene in Escherichia coli (BL21 DE3) yielded largely insoluble protein. The H109R variant is expressed in lower amounts compared to the wild-type enzyme
expressed in Escherichia coli BL21(DE3) cells as a C-terminally 6-His-tagged fusion protein which is predominantly in its apo form
expression in Escherichia coli
overexpression in Escherichia coli strain JM109 as N-terminally His-tagged enzyme being equally distributed in the soluble fraction and the insoluble inclusion body fraction
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screening of a cDNA library prepared in Escherichia coli from rat liver polyA RNA in the plasmid vector peD-X