1.5.99.12: cytokinin dehydrogenase This is an abbreviated version! For detailed information about cytokinin dehydrogenase, go to the full flat file .
Reaction
N6-dimethylallyladenine +
acceptor +
H2O =
adenine +
3-methylbut-2-enal +
reduced acceptor
Synonyms AtCKX2, AtCKX3, AtCKX6, AtCKX7, CKO, CKO1, CKX, CKX1, CKX10, CKX12, CKX2, CKX2-2, CKX3, CKX4, CKX4a, CKX5, CKX6, CKX7, CKX8, CKX9, cytokinin dehydrogenase, cytokinin dehydrogenase 1, cytokinin dehydrogenase CKX1, cytokinin dehydrogenase CKX10, cytokinin oxidase, cytokinin oxidase/dehydrogenase, DhCKX, HvCKX1, isopentenyladenosine oxidase, More, N6-(D2-isopentenyl)adenosine oxidase, N6-dimethylallyladenine dehydrogenase, N6-isopentenylaldenine oxidase, OsCKX2, PsCKX1, PsCKX2, VIT_00s0252g00040, VIT_00s2520g00010, VIT_04s0008g01880, VIT_07s0005g05960, VIT_07s0005g06010, VIT_11s0016g02110, VIT_13s0158g00320, VIT_18s0001g13200, zeatin oxidase, ZmCKO1, ZmCKX1
ECTree
Reaction
Reaction on EC 1.5.99.12 - cytokinin dehydrogenase
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N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor
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N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor
reaction might also be catalyzed by a different enzyme in Dictyostelium discoideum, since no related sequence has been found
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N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor
reaction scheme and mechanism, reduction of N6-(2-isopentenyl)adenine is rapidly performed, the reaction with O2 is slowly
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor
reaction scheme and mechanism, the substrate displays a plug-into-socket binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin, bipartite architecture of the catalytic centre, which consists of a funnel-shaped region on the protein surface and an internal cavity lined by the flavin ring, the 3 active site regions are connected by a pore of about 4 A diameter
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor
Asp162 and Glu275 involved in substrate binding