1.5.99.15: dihydromethanopterin reductase (acceptor)

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For detailed information about dihydromethanopterin reductase (acceptor), go to the full flat file.

Reaction

Synonyms

AfpA, archaeal-flavoprotein-like flavoprotein, Bxe_B2440, dihydromethanopterin reductase, Dmr, DmrB, DmrX, H2MPT reductase, methylene H4MPT dehydrogenase B, MJ0208, MM1854, MtdB

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.99 With unknown physiological acceptors

1.5.99.15 dihydromethanopterin reductase (acceptor)

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Results	in table
129	AA Sequence
7	Cloned(Commentary)
14	Cofactor
2	Crystallization (Commentary)
15	General Information
1	General Stability
4	Metals/Ions
1	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
14	Organism
4	Pathway
2	pH Optimum
6	Purification (Commentary)
2	Reaction
10	Reference
1	Renatured (Commentary)
1	Storage Stability
19	Substrates and Products (Substrate)
7	Subunits
30	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
4	Temperature Stability [°C]

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Cloned on EC 1.5.99.15 - dihydromethanopterin reductase (acceptor)

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expressed in Escherichia coli BL21 (DE3)-RIL cells

Paraburkholderia xenovorans

Q13QT8

742870

expression in Escherichia coli

3 entries

gene dmrB, recombinant expression of N-terminally His6 -tagged enzyme in Escherichia coli strain BL21DE3 RIL

Paraburkholderia xenovorans

Q13QT8

742870

gene dmrX, recombinant expression of His6-tagged enzyme in Escherichia coli strain BE100 [BL21(DE3) RIL]

Methanosarcina mazei

Q8PVV3

742781

gene dmrX, recombinant expression of the codon-optimized gene encoding His6-tagged enzyme in Escherichia col strain BE100 [BL21(DE3) RIL]

Methanocaldococcus jannaschii

Q57661

742781