1.5.99.B2: proline dehydrogenase (acceptor)
This is an abbreviated version!
For detailed information about proline dehydrogenase (acceptor), go to the full flat file.
Reaction
Synonyms
APE_1267.1, dye-linked L-proline dehydrogenase, L-proDH, L-proline dehydrogenase, L-proline: FAD oxidoreductase, L-proline:FAD oxidoreductase, LPDH, PDH, PDH1, PDH2, PF1246, PF1798, PH1364, PH1751, PIG6, POX, Pro dehydrogenase, PRODH, ProDH1, ProDH2, proline dehydrogenase, proline oxidase, prub, PutA, TK0117, TK0122, TPpdhB, TPpdhB2, TtProDH
ECTree
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Purification
Purification on EC 1.5.99.B2 - proline dehydrogenase (acceptor)
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DEAE Toyopearl 650M column chromatography and Superdex 200 gel filtration
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HisTrap column chromatography and Hitrap Q column chromatography
Ni-chelating column chromatography
Ni2+-charged chelating Sepharose column chromatography and Sephacryl S-300 gel filtration
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recombinant C-terminally His6-tagged wild-type and mutant enzymes from Mycobacterium smegmatis mc24517 cells by nickel affinity chromatography
recombinant enzyme
recombinant enzyme via refolding, reconstitution and two-phase partitioning in an optimized aqueous two-phase system, containing 14% w/w PEG-1000 and 12% w/w Na2CO3 at pH 8.0, from Escherichia coli strain BL21 (DE3) plysS inclusion bodies in one step, method optimization and evaluation, overview
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