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1.6.2.4: NADPH-hemoprotein reductase

This is an abbreviated version!
For detailed information about NADPH-hemoprotein reductase, go to the full flat file.

Word Map on EC 1.6.2.4

Reaction

NADPH
+
oxidized hemoprotein
=
NADP+
 +
reduced hemoprotein
 +
H+

Synonyms

107366505, aldehyde reductase (NADPH-dependent), ATR2, CPR, CPR I, CPR-X1, CPR-X2, CPR1, CPR2, CYPOR, cytochrome c reductase (reduced nicotinamide adenine dinucleotide phosphate, NADPH, NADPH-dependent), cytochrome P450 NADPH reductase, cytochrome P450 oxidoreductase, cytochrome P450 reductase, cytochrome P450-diflavin reductase, dihydroxynicotinamide adenine dinucleotide phosphate-cytochrome c reductase, FAD-cytochrome c reductase, ferrihemprotein P450 reductase, flavocytochrome P450 BM3, NAD(P)H cytochrome P450 reductase, NADP-cytochrome c reductase, NADP-cytochrome P-450 reductase, NADP-cytochrome reductase, NADPH cytochrome P450 reductase, NADPH dependent cytochrome P450 reductase, NADPH P450 oxidoreductase, NADPH P450 reductase, NADPH-CPR, NADPH-CYP reductase, NADPH-CYP450 reductase, NADPH-cytochrome c oxidoreductase, NADPH-cytochrome c reductase, NADPH-cytochrome p-450 reductase, NADPH-cytochrome P450 (CYP) oxidoreductase, NADPH-cytochrome P450 oxidoreductase, NADPH-cytochrome P450 reductase, NADPH-cytochrome P450 reductase 2, NADPH-dependent cytochrome c reductase, NADPH-dependent cytochrome P450 reductase, NADPH-dependent P450 reductase, NADPH-ferricytochrome c oxidoreductase, NADPH-ferrihemoprotein reductase, NADPH-P450 reductase, NADPH: cytochrome P450 oxidoreductase, NADPH: cytochrome P450 reductase, NADPH:CYP oxidoreductase, NADPH:cytochrome P450 oxidoreductase, NADPH:cytochrome P450 reductase, NADPH:ferrihemoprotein oxidoreductase, NCP1, neotetrazolin reductase, NPR, NP_000932, P-450 reductase, P450 BM3, P450 oxidoreductase, P450 Red, P450 reductase, P450R, P450REd, POR, RedA, reduced nicotinamide adenine dinucleotide phosphate-cytochrome c reductase, reductase, cytochrome c (reduced nicotinamide adenine dinucleotide phosphate), SbCPR2, TcCPR-A, TcCPR-B, TcCPR-C, TPNH-cytochrome c reductase, TPNH2 cytochrome c reductase, WsCPR1, WsCPR2

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.2 With a heme protein as acceptor
                1.6.2.4 NADPH-hemoprotein reductase

Purification

Purification on EC 1.6.2.4 - NADPH-hemoprotein reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2',5'-ADP agarose affinity column chromatography and DEAE resin column chromatography
-
2',5'-ADP Sepharose column chromatography
2’,5’-ADP Sepharose affinitycolumn chromatography and DEAE anion exchange column chromatography
-
anion exchange chromatography and affinity chromatography
-
chromatography in DEAE-cellulose, DEAE-Sephadex A-50 and hydroxylapatite
-
chromatography on 2',5'-ADP agarose
column chromatography on 2',5'-ADP-Sepharose, phospholipid composition relatively enriched in L-alpha-1,2-diacyl-sn-glycero-3-phosphoserine and L-alpha-1,2-diacyl-sn-glycero-3-phosphoinositol
-
column chromatography on DEAE-cellulose and 2',5'-ADP-Sepharose 4B
-
column chromatography on DEAE-cellulose and affinity chromatography on cytochrome-c-Sepharose 4B
-
column chromatography on phenyl-sepharose CL-4B, DEAE cellulose, hydroxylapatite and Sephadex G-150
-
column chromatography on Sephadex G-100 and DEAE-cellulose
column chromatography on Sephadex G-150 and DE-52
-
DEAE-cellulose and 2',5'-ADP-agarose column chromatography
-
DEAE-cellulose and Sephadex G-100 column chromatography
-
DEAE-cellulose column chromatography
-
DEAE-cellulose followed by sequential chromatography on Sephadex G-150, DEAE-cellulose and hydroxylapatite
-
DEAE-Sephacel and hydroxylapatite column chromatography
-
DEAE-Sephadex gel filtration and 2',5'-ADP Sepharose 4B column chromatography
-
DEAE-Sepharose column chromatography
-
expressed in Escherichia coli
-
expressed in Escherichia coli, column chromatography on 2',5'-ADP-agarose
-
extraction of native enzyme from liver microsomes by Brij 98 solubilization and isolation of detergent-resistant membranes by sucrose gradient centrifugation
-
FAD-depleted enzyme
-
glutathione-Sepharose column chromatography
homogeneity, chromatographic methods (DEAE-Sepharose, Q-Sepharose and hydroxyapatite resins)
liver microsomes, column chromatography on Sephadex G-150 and hydroxylapatatite
-
mitochondria
-
Ni-NTA agarose column chromatography and Superdex 200 gel filtration
-
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex G25 gel filtration, the purified enzyme readily loses its flavin cofactors
-
Ni-NTA column chromatography, gel filtration
-
Ni-NTA resin column chromatography
Ni-NTA resin column chromatography, and Superdex 200 gel filtration
purified 120fold by a combination of ion exchange chromatography and gel filtration and 745fold by affinity chromatography on 2',5'-ADP-Sepharose 4B
-
Q-Sepharose column chromatography and Superdex 200 gel filtration
-
recombinant
-
recombinant enzyme
-
recombinant enzyme from Escherichia coli strain DH5alpha
recombinant enzyme, membrane-bound
recombinant enzymes using His-tag
-
recombinant N-terminally truncated and His6-tagged enzyme, lacking the putative membrane anchor, from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to over 98% purity
recombinant N-terminally truncated and His6-tagged protein from Escherichia coli Rosetta (DE3) cells to over 90% purity
recombinant NADPH-cytochrome c oxidoreductase lacking the first 55 amino acid residues (DELTA55AnCY) and recombinant mutant enzyme L86F/L219F/P456ADELTA55AnCY
-
recombinant protein, by Ni-NTA affinity chromatography
-
recombinant protein, using Ni2+-affinity chromatography
recombinant truncated mutant from Escherichia coli strain C41(DE3) by aanion exchange and 2',5'-ADP-Sepharose
sequential chromatography on DE 52 cellulose and 2',5'-ADP Sepharose
-
single mutation K56Q
-
single-step procedure involving affinity chromatography on agarose-hexane-adenosine 2',5'-diphosphate or two-column procedure involving chromatography on n-octylamino-Sepharose 4B followed by chromatography on 2',5'-ADP
-
solubilization with 0.2% Triton X-100, gel filtration on Sephacryl S-300 and affinity chromatography on 2',5'-ADP agarose
-
solubilization with lipase, column chromatography on Sephadex G-100 superfine, DEAE-cellulose and Sephadex G-200 superfine
-
solubilization with sodium cholate followed by column chromatography on phenyl-Sepharose CL-4B and DEAE-cellulose
-
trypsin digestion followed by Sephadex filtration and column chromatography on DEAE-cellulose
-
using column chromatography on adenosine 2',5'-biphosphate-Sepharose, followed by high-performance anion-exchange chromatography
-
using column chromatography on DEAE-Sepharose CL 6B and affinity chromatography on a 2',5'-ADP Sepharose 4B column
-
WsCPR1, recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography, cleavage of the tag by thrombin, and elimination of thrombin by benzymedene beads
WsCPR2, recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography, cleavage of the tag by thrombin, and elimination of thrombin by benzymedene beads