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1.6.5.4: monodehydroascorbate reductase (NADH)

This is an abbreviated version!
For detailed information about monodehydroascorbate reductase (NADH), go to the full flat file.

Word Map on EC 1.6.5.4

Reaction

NADH
+
H+
+ 2 monodehydroascorbate =
NAD+
+ 2 ascorbate

Synonyms

12-oxophytodienoate reductase 3, AFR reductase, AFR-reductase, AFRR, ascorbate free radical reductase, ascorbate free-radical reductase, ascorbic free radical reductase, At1g63940, At3g09940, AtMDAR1, defensin, MDA, MDA reductase, MDAR, MDAR-OX, MDAR1, MDAR2, MDAR5, MDAsA reductase (NADPH), MDHA, MDHAR, MDHAR1, MDHAR2, MDHAR3, MDHAR6, metallothionein-like type 1 protein, monodehydroascorbate radical reductase, monodehydroascorbate reductase, monodehydroascorbate reductase 2, monodehydroascorbate reductase 4, More, MT-2, MT1K, NADH-semidehydroascorbate oxidoreductase, NADH:AFR oxidoreductase, NADH:ascorbate radical oxidoreductase, NADH:semidehydroascorbic acid oxidoreductase, NEC3, Nectarin III, OPR3, Os09g0567300, peroxisomal monodehydroascorbate reductase, PpMDHAR1, PpMDHAR2, PpMDHAR3, SDA reductase, semidehydroascorbate reductase, Solyc09g009390, SOR, SPD1, thioredoxin H2, Trx h2

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.5 With a quinone or similar compound as acceptor
                1.6.5.4 monodehydroascorbate reductase (NADH)

Inhibitors

Inhibitors on EC 1.6.5.4 - monodehydroascorbate reductase (NADH)

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
10 mM, 16% inhibition
1-(2-thenoyl)-3,3,3-trifluoracetone
-
0.3 mM, about 10% inhibition
2,2'-dipyridyl
2,4,6-Trinitrobenzenesulfonic acid
-
1 mM, 54% inhibition
2-Iodoacetamide
2-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-amino]ethanesulfonic acid
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
8-hydroxyquinoline
-
10 mM, 15% inhibition
beta-chloromercuribenzoate
PpMDHAR1 is almost completely inactivated after a 20 min preincubation with 0.25 mM beta-chloromercuribenzoate. At 0.05 mM the enzyme retains 11% of the initial activity.
chloromercuribenzoate
at 0.05 mM PpMDHAR3 retains 80% of the initial activity; PpMDHAR2 is almost completely inactivated after a 20 min preincubation with 0.25 mM chloromercuribenzoate. At 0.05 mM the enzyme retains 14% of the initial activity. The C69A mutant is more resistant to the inhibitory effects of chloromercuribenzoate than the wild type PpMDHAR2 protein
citrate
-
1 mM, about 10% inhibition
Cu2+
-
0.5 mM, complete inhibition
diacetyl
-
1 mM, 55% inhibition
dicoumarol
diethyl dicarbonate
-
0.1 mM, 62% inhibition
EDTA
-
10 mM, 11% inhibition
Hg2+
-
0.5 mM, complete inhibition
Imidazole buffer
-
-
-
Insulin
-
inhibition of the enzyme in plasma membrane
-
iodoacetamide
iodoacetate
lectin
-
ConA, WGA and LFA
-
Mersalyl
NaN3
-
16 mM, 16% inhibition
p-chloromercuribenzoate
peroxynitrite
exclusively nitrates residues Tyr213, Try292, and Tyr345. Tyr345 is found at 3.3 A of His313, which is involved in the NADP-binding site. Nitration of residue Tyr345 is responsible for inhibition
phenyl mercuric acetate
-
0.1 mM, 85% inhibition
Phenylglyoxal
-
100 mM, 46% inhibition
phosphate
-
-
pyridoxal 5'-phosphate
-
1 mM, 46% inhibition
S-nitrosoglutathione
0.5 and 2 mM, 58% and 65% inhibition, respectively
sodium diethyldithiocarbaminate
-
1 mM, about 10% inhibition
Thenoyltrifluoroacetone
-
0.15 mM, complete inhibition
thiol-modifiying reagents
-
inhibition at the step of FAD reduction by NADH producing a charge-transfer complex
-
Trinitromethane
-
10 mM, 11% inhibition
additional information
-