1.6.99.1: NADPH dehydrogenase

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For detailed information about NADPH dehydrogenase, go to the full flat file.

Reaction

Synonyms

2-cyclohexen-1-one, dehydrogenase, reduced nicotinamide adenine dinucleotide phosphate, diaphorase, dihydronicotinamide adenine dinucleotide phosphate dehydrogenase, EBP, Estrogen-binding protein, NADP-d, NADPH diaphorase, NADPH-d, NADPH-dehydrogenase, NADPH-dependent cyclohexenone reductase, NADPH-dependent diaphorase, NADPH-diaphorase, NADPH-methemoglobin reductase, NADPH2-dehydrogenase, NCR, nicotinamide adenine dinucleotide phosphate diaphorase, nitric oxide synthase, old yellow enzyme, Old yellow enzyme homolog, OYE, OYE1, OYE2, OYE3, reduced nicotinamide adenine dinucleotide phosphate dehydrogenase, SYE4, TPNH dehydrogenase, TPNH-diaphorase, triphosphopyridine diaphorase, triphosphopyridine nucleotide diaphorase, YqjM

ECTree

     1 Oxidoreductases

         1.6 Acting on NADH or NADPH

             1.6.99 With unknown physiological acceptors

                1.6.99.1 NADPH dehydrogenase

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Results	in table
3083	AA Sequence
3	Application
1	CAS Registry Number
7	Cloned(Commentary)
34	Cofactor
4	Crystallization (Commentary)
13818	Disease/ Diagnostics
1	Expression
4	General Information
10	Inhibitors
1	Ki Value [mM]
23	KM Value [mM]
4	Localization
2	Metals/Ions
17	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
316	Organism
87	PDB ID
2	pH Optimum
2	Protein Variants
6	Purification (Commentary)
3	Reaction
3	Reaction Type
45	Reference
36	Source Tissue
66	Substrates and Products (Substrate)
11	Subunits
53	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
23	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.6.99.1 - NADPH dehydrogenase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Saccharomyces pastorianus

-

392784, 392803

purified enzyme, hanging drop vapor diffusion method, mixing of 0.002 ml of protein solution containing 14 mg/mL in 25 mM Tris-HCl, pH 8.0, containing 100 mM NaCl, and 1 mM 2-mercaptoethanol, with 0002 ml of 28% w/v PEG 1500, and 0.3 M ammonium fluoride, equilibration against 0.5 ml of reservoir solution, 19°C, 10 days, crystalline forms I and II, X-ray diffraction structure determination and analysis at 1.27 A and 2.0 A resolution, respectively

Trypanosoma cruzi

Q2TJB8

721342

purified recombinant enzyme in a ternary complex with inhibitor 12-oxophytodienoate and FMN, X-ray diffraction structure determination and analysis at 2.05 A resolution

Trypanosoma cruzi

-

725313