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1.7.1.3: nitrate reductase (NADPH)

This is an abbreviated version!
For detailed information about nitrate reductase (NADPH), go to the full flat file.

Word Map on EC 1.7.1.3

Reaction

nitrite
+
NADP+
 +
H2O
=
nitrate
 +
NADPH
 +
H+

Synonyms

assimilatory NADPH-nitrate reductase, assimilatory NADPH:nitrate reductase, Assimilatory nitrate reductase, assimilatory reduced nicotinamide adenine dinucleotide phosphate-nitrate reductase, EC 1.6.6.3, EC 1.7.99.4, MSMEG_2837, NADPH-dependent nitrate reductase, NADPH-nitrate reductase, NADPH2:nitrate oxidoreductase, NADPH:nitrate reductase, NADPH:NR, NaR1, narB, nit-3, nitrate reductase, nitrate reductase (NADPH), nitrate reductase (reduced nicotinamide adenine dinucleotide phosphate), nitrate reductase [NADPH], NR, triphosphopyridine nucleotide-nitrate reductase

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.3 nitrate reductase (NADPH)

Specific Activity

Specific Activity on EC 1.7.1.3 - nitrate reductase (NADPH)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0001
0.003
ectomycchorizae
0.004 - 0.074
-
various temperature-sensitive mutants
0.012
-
wild-type enzyme, anaerobic conditions, presence of ammonium
0.013
-
wild-type enzyme, aerobic conditions, presence of nitrate
0.018
-
wild-type enzyme, anaerobic conditions, presence of nitrate
0.019
-
wild-type enzyme, anaerobic conditions, presence of nitrate and ammonium
0.02
-
activity under aerobic conditions in presence of NH4+
0.063
-
nit-1 mutant
0.074
-
wild type
0.1
-
activity under aerobic conditions
0.3
-
activity under anaerobic conditions
0.37
-
activity under anaerobic conditions in presence of NH4+
177.1
-
purified native enzyme, substrate nitrate, pH 7.2, 37°C
4 - 16.1
-
at different stages of purification
4.32
-
purified recombinant holo-enzyme, substrate nitrate, pH 7.5, temperature not specified in the publication
additional information