1.7.2.3: trimethylamine-N-oxide reductase
This is an abbreviated version!
For detailed information about trimethylamine-N-oxide reductase, go to the full flat file.
Reaction
+ 2 (ferricytochrome c)-subunit + = + 2 (ferrocytochrome c)-subunit + 2 H+
Synonyms
EC 1.6.6.9, More, reductase, trimethylamine N-oxide, TMAO reductase, TOR, TorA, TorD, torECA gene product, TorECAD, TorZ, trimethylamine N-oxide reductase, trimethylamine oxide reductase
ECTree
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Cloned
Cloned on EC 1.7.2.3 - trimethylamine-N-oxide reductase
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amplification of the structural torA gene encoding the enzyme and iits flanking regions using PCR techniques, the torA gene is the third gene of a TMAO-inducible operon, torECAD, encoding the TMAO respiratory components, the torC gene encodes a pentahemic c-type cytochrome, likely to be involved in electron transfer to the TorA terminal reductase, the role of the torE gene is unknown, torD gene encodes the TorD protein, a cytoplasmic protein involved in the folding process of the TorA precursor protein
gene torA, the torCAD operon encodes the Tor system induced in the presence of trimethylamine oxide mainly during exponential phase
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gene torD, DNA sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) as His-tagged protein
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GFP-tagged enzyme expression, the genome sequence of Vibrio fischeri reveals three putative TMAO reductase operons, transcriptional regulation, overview
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the torECA promoter, and to a lesser extent the torYZ and dmsABC promoters, are active during symbiotic colonization of juvenile Euprymna scolopes, transcriptional regulation, overview
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the trimethylamine N-oxide reductase respiratory system is encoded by the torECAD operon, a three-gene cluster, torSTR, encoding a complex two-component regulatory system is present downstream of the torECAD operon, introduction of the torSTR gene cluster into Escherichia coli shows that this regulatory gene cluster is involved in trimethylamine N-oxide induction of the torE promoter, the torR operator site is required for induction of the tor structural promoter, TorS of Escherichia coli , the trimethylamine N-oxide sensor of Escherichia coli is able to transphosphorylate TorR of Shewanella oneidensis, the trimethylamine N-oxide response regulator of Shewanella oneidensis
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