1.7.3.3: factor-independent urate hydroxylase
This is an abbreviated version!
For detailed information about factor-independent urate hydroxylase, go to the full flat file.
Word Map on EC 1.7.3.3
-
1.7.3.3
-
hyperuricemia
-
peroxisomal
-
xanthine
-
allopurinol
-
gout
-
purine
-
catalase
-
allantoin
-
biosensors
-
electrode
-
hematologic
-
creatinine
-
oxonic
-
febuxostat
-
allantoinase
-
hyperkalemia
-
hypoxanthine
-
prophylaxis
-
flavus
-
hyperphosphatemia
-
urate-lowering
-
ureide
-
methemoglobinemia
-
pegylated
-
amperometric
-
uricosuric
-
hypocalcemia
-
tophi
-
benzbromarone
-
hominoid
-
phosphotungstate
-
microbodies
-
hypouricemic
-
probenecid
-
utilis
-
pimecrolimus
-
pharmacology
-
medicine
-
analysis
-
synthesis
-
weight-based
-
nodule-specific
-
miocene
-
drug development
- 1.7.3.3
- hyperuricemia
- peroxisomal
- xanthine
- allopurinol
- gout
- purine
- catalase
- allantoin
-
biosensors
-
electrode
-
hematologic
- creatinine
-
oxonic
- febuxostat
- allantoinase
- hyperkalemia
- hypoxanthine
-
prophylaxis
- flavus
- hyperphosphatemia
-
urate-lowering
-
ureide
- methemoglobinemia
-
pegylated
-
amperometric
-
uricosuric
-
hypocalcemia
-
tophi
- benzbromarone
-
hominoid
-
phosphotungstate
- microbodies
-
hypouricemic
- probenecid
- utilis
- pimecrolimus
- pharmacology
- medicine
- analysis
- synthesis
-
weight-based
-
nodule-specific
-
miocene
- drug development
Reaction
Synonyms
AaUO, AgUOX, dHU-wPU, ELITEK, Fasturtec, MVSM, N-35, Nodule specific uricase, Nodulin 35, Nodulin 35 homolog, Non-symbiotic uricase, oxidase, urate, Pucl, Rasburicase, Uaz, Uox, Urate oxidase, urate oxidoreductase, UriA, uric acid oxidase, uricase, uricase II, Uricoenzyme, Uricozyme
ECTree
Advanced search results
Specific Activity
Specific Activity on EC 1.7.3.3 - factor-independent urate hydroxylase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
15
pH not specified in the publication, temperature not specified in the publication
2.49
-
urate oxidase including p-azido-L-phenylalanine instead of Phe at position 281, in 0.1 M borate, pH 8.4
2.99
mutant A89T/G91A7V92M/H245L/E252A/M253I/R291K/A296V/A301S/K303R, pH 8.6, 25°C
4.91
mutant H245L/E252A/M253I/R291K/A296V/A301S/K303R, pH 8.6, 25°C
8.26
-
urate oxidase including p-azido-L-phenylalanine instead of Phe at position 170, in 0.1 M borate, pH 8.4
additional information
-
among the parameters investigated in shaking flask cultures, the pH value of medium and inoculum size has great influence on the recombinant uricase production, the maximum extracellular uricase yield of 2.6 U/ml is obtained in shaking flask culture
additional information
-
at pH 5.5, the extracellular uricase production reaches top of 7.5 U/ml at 58 h, when fermentation is performed at pH 6.5 for 62 h, 14.5 U/ml of extracellular uricase and 23.3 U/ml of intracellular uricase are produced, the total specific uricase production at pH 6.5 is 1.7times of that at pH 5.5
additional information
-
in high density fermentation in YPG medium at 37°C, extracellular uricase activity increases significantly during the first 40 h, highest extracellular uricase level of 52.3 U/ml is obtained after 58 h of induction, as well as the intracellular activity of 60.3 U/ml, after 86 h of fermentation and 58 h of induction, a total uricase activity of 112600 U/l is obtained, the extracellular and intracellular yields of uricase in high cell density fermentation increased by 3.7fold and 3.5fold compared with the batch fermentation
additional information
-
the combined use of fed-batch culture and pH-controlled strategy increases the expression level of uricase significantly, the extracellular uricase production of 52.3 U/ml (approximately 2.1 g/l of protein) is obtained, which is much higher than that produced by recombinant Escherichia coli strains
additional information
DQ887577
enzyme activity is 0.06 U/ml at pH 4.0
additional information
-
enzyme activity is 0.06 U/ml at pH 4.0
additional information
DQ887577
enzyme activity is 0.09 U/ml at pH 4.5
additional information
-
enzyme activity is 0.09 U/ml at pH 4.5
additional information
DQ887577
enzyme activity is 0.11 U/ml at pH 5.0
additional information
-
enzyme activity is 0.11 U/ml at pH 5.0
additional information
DQ887577
enzyme activity is 0.21 U/ml with potassium nitrate as nitrogen source
additional information
-
enzyme activity is 0.21 U/ml with potassium nitrate as nitrogen source
additional information
DQ887577
enzyme activity is 0.22 U/ml at pH 10.0
additional information
-
enzyme activity is 0.22 U/ml at pH 10.0
additional information
DQ887577
enzyme activity is 0.31 U/ml with ammonium sulfate as nitrogen source
additional information
-
enzyme activity is 0.31 U/ml with ammonium sulfate as nitrogen source
additional information
DQ887577
enzyme activity is 0.32 U/ml with sodium glutamate as nitrogen source
additional information
-
enzyme activity is 0.32 U/ml with sodium glutamate as nitrogen source
additional information
DQ887577
enzyme activity is 0.37 U/ml at pH 5.5
additional information
-
enzyme activity is 0.37 U/ml at pH 5.5
additional information
DQ887577
enzyme activity is 0.42 U/ml at pH 9.5
additional information
-
enzyme activity is 0.42 U/ml at pH 9.5
additional information
DQ887577
enzyme activity is 0.45 U/ml with citric acid as carbon source
additional information
-
enzyme activity is 0.45 U/ml with citric acid as carbon source
additional information
DQ887577
enzyme activity is 0.46 U/ml with glucose as carbon source
additional information
-
enzyme activity is 0.46 U/ml with glucose as carbon source
additional information
DQ887577
enzyme activity is 0.46 U/ml with peptone as nitrogen source
additional information
-
enzyme activity is 0.46 U/ml with peptone as nitrogen source
additional information
DQ887577
enzyme activity is 0.47 U/ml at pH 6.0
additional information
-
enzyme activity is 0.47 U/ml at pH 6.0
additional information
DQ887577
enzyme activity is 0.47 U/ml with lactose as carbon source
additional information
-
enzyme activity is 0.47 U/ml with lactose as carbon source
additional information
DQ887577
enzyme activity is 0.49 U/ml with starch as carbon source
additional information
-
enzyme activity is 0.49 U/ml with starch as carbon source
additional information
DQ887577
enzyme activity is 0.57 U/ml at pH 9.0
additional information
-
enzyme activity is 0.57 U/ml at pH 9.0
additional information
DQ887577
enzyme activity is 0.57 U/ml with soybean flour as nitrogen source
additional information
-
enzyme activity is 0.57 U/ml with soybean flour as nitrogen source
additional information
DQ887577
enzyme activity is 0.63 U/ml at pH 8.5
additional information
-
enzyme activity is 0.63 U/ml at pH 8.5
additional information
DQ887577
enzyme activity is 0.65 U/ml with beef extract as nitrogen source
additional information
-
enzyme activity is 0.65 U/ml with beef extract as nitrogen source
additional information
DQ887577
enzyme activity is 0.67 U/ml with sucrose as carbon source
additional information
-
enzyme activity is 0.67 U/ml with sucrose as carbon source
additional information
DQ887577
enzyme activity is 0.69 U/ml with yeast extract as nitrogen source
additional information
-
enzyme activity is 0.69 U/ml with yeast extract as nitrogen source
additional information
DQ887577
enzyme activity is 0.72 U/ml at pH 8.0
additional information
-
enzyme activity is 0.72 U/ml at pH 8.0
additional information
DQ887577
enzyme activity is 0.78 U/ml with maize milk as nitrogen source
additional information
-
enzyme activity is 0.78 U/ml with maize milk as nitrogen source
additional information
DQ887577
enzyme activity is 0.98 U/ml at pH 6.5
additional information
-
enzyme activity is 0.98 U/ml at pH 6.5
additional information
DQ887577
enzyme activity is 1.00 U/ml at pH 7.5
additional information
-
enzyme activity is 1.00 U/ml at pH 7.5
additional information
DQ887577
enzyme activity is 1.25 U/ml at pH 7.0
additional information
-
enzyme activity is 1.25 U/ml at pH 7.0
additional information
DQ887577
when the strain is cultured at 30°C at pH 7.0 for 30-36 h with of 0.6% corn steep liquor as nitrogen source, the uricase activity peaks at 1.25 U/ml
additional information
-
when the strain is cultured at 30°C at pH 7.0 for 30-36 h with of 0.6% corn steep liquor as nitrogen source, the uricase activity peaks at 1.25 U/ml
additional information
-
rasburicase causes enzymatic degradation of uric acid within blood, plasma and serum samples at room temperature, the genetic absence of this molecule in humans and its proteic nature together with poor accuracy in purifi cation and a slow production process confer a high immunogenicity to the compound, leading to elevated rate of hypersensivity reactions
additional information
-
rasburicase does not interact with allopurinol, cytarabine, methylprednisolone, methotrexate, mercaptopurine, thioguanine, etoposide, daunorubicin, cyclophosphamide, or vincristine
additional information
-
rasburicase maintains the same mechanism of action as the non-recombinant form of urate oxidase, but simply shows a significantly lower reaction rate
additional information
-
repeated use of rasburicase increases risk of hypersensitivity reactions: skin rashes (1.4%), urticaria, bronchospasm (1%), dyspnea, hypoxemia, and anaphylactic shock (1%)