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1.7.3.3: factor-independent urate hydroxylase

This is an abbreviated version!
For detailed information about factor-independent urate hydroxylase, go to the full flat file.

Word Map on EC 1.7.3.3

Reaction

Urate
+
O2
+
H2O
=
5-hydroxyisourate
+
H2O2

Synonyms

AaUO, AgUOX, dHU-wPU, ELITEK, Fasturtec, MVSM, N-35, Nodule specific uricase, Nodulin 35, Nodulin 35 homolog, Non-symbiotic uricase, oxidase, urate, Pucl, Rasburicase, Uaz, Uox, Urate oxidase, urate oxidoreductase, UriA, uric acid oxidase, uricase, uricase II, Uricoenzyme, Uricozyme

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.3 With oxygen as acceptor
                1.7.3.3 factor-independent urate hydroxylase

Specific Activity

Specific Activity on EC 1.7.3.3 - factor-independent urate hydroxylase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.067
-
wild-type, pH 8.9, 25°C
10.38
mutant enzyme N289C, at pH 8.0 and 30°C
10.45
wild type enzyme, at pH 8.0 and 30°C
10.5
-
in the cell lysate
11.3
mutant enzyme V144A/Y319R, at pH 9.2 and 25°C
11.4
mutant enzyme V144A, at pH 9.2 and 25°C
13.3
-
hypocotyls, 4 d old
15
pH not specified in the publication, temperature not specified in the publication
15.3
-
female
15.38
mutant enzyme A132C/A225C, at pH 8.0 and 30°C
15.4
-
roots, 4 d old
16.33
-
natural uricase, in 0.1 M borate, pH 8.4
1790
-
nodules, 21 d old
18
-
crude extract, pH 8.0, 37°C
2.49
-
urate oxidase including p-azido-L-phenylalanine instead of Phe at position 281, in 0.1 M borate, pH 8.4
2.67
-
recombinant strain overexpressing the enzyme, pH 8.9, 25°C
2.99
mutant A89T/G91A7V92M/H245L/E252A/M253I/R291K/A296V/A301S/K303R, pH 8.6, 25°C
21.5
-
after DEAE Sepharose FF chromatography
25.7
-
after Phenyl-Sepharose FF chromatography
27
-
after HiLoad 26/60 Superdex 75 gel filtration
3.74
-
cotyledons, 4 d old
38.4
recombinant protein, pH 8.0, 37°C
39
-
after 2.1fold purification, pH 8.0, 37°C
4.22
wild-type, pH 8.6, 25°C
4.91
mutant H245L/E252A/M253I/R291K/A296V/A301S/K303R, pH 8.6, 25°C
5.32
-
purified enzyme
5.36
-
soluble cell lysate, at pH 9.0 and 42°C
5.68
mutant A296V, pH 8.6, 25°C
5.94
mutant R291K/A296V/A301S/K303R, pH 8.6, 25°C
50.54
-
after 9.43fold purification, at pH 9.0 and 42°C
6.8
wild-type, pH 9.2, 27°C
6.85
-
-
7.7
-
crude extract, at pH 7.5 and 30°C
7.88
mutant enzyme S136C, at pH 8.0 and 30°C
8.26
-
urate oxidase including p-azido-L-phenylalanine instead of Phe at position 170, in 0.1 M borate, pH 8.4
8.3
mutant L1711I/Y182F/Y187F/A193S, pH 9.2, 27°C
87
-
after 11.3fold purification, at pH 7.5 and 30°C
9
wild type enzyme, at pH 9.2 and 25°C
9.2
mutant enzyme Y319R, at pH 9.2 and 25°C
9.35
-
male rats
9.38
mutant enzyme D288C, at pH 8.0 and 30°C
9.75
mutant enzyme I27C/N289C, at pH 8.0 and 30°C
additional information