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1.7.3.3: factor-independent urate hydroxylase

This is an abbreviated version!
For detailed information about factor-independent urate hydroxylase, go to the full flat file.

Word Map on EC 1.7.3.3

Reaction

Urate
+
O2
 +
H2O
=
5-hydroxyisourate
 +
H2O2

Synonyms

AaUO, AgUOX, dHU-wPU, ELITEK, Fasturtec, MVSM, N-35, Nodule specific uricase, Nodulin 35, Nodulin 35 homolog, Non-symbiotic uricase, oxidase, urate, Pucl, Rasburicase, Uaz, Uox, Urate oxidase, urate oxidoreductase, UriA, uric acid oxidase, uricase, uricase II, Uricoenzyme, Uricozyme

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.3 With oxygen as acceptor
                1.7.3.3 factor-independent urate hydroxylase

pH Stability

pH Stability on EC 1.7.3.3 - factor-independent urate hydroxylase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
45°C, 30 min, 30% loss of activity
394146
11
-
25°C, 18 h, about 60% loss of activity
701416
12
-
22°C, 30 min, 55% loss of activity
394156
6 - 10
-
the purified enzyme remains stable over a wide range of pH between 6.0-10.0 for 2 h
764830
6 - 11
-
35°C, 1 h, stable
394144
6.5 - 10.5
-
22°C, 30 min, immobilized enzyme, stable
394156
7
UOX is deactivated at different protein concentrations at 45°C in 20 mM phosphate containing 0.15 M NaCl, 0.01 mg/ml UOX is deactivated much faster than its counterparts at concentrations of 0.1 and 1.0 mg/ml
700631
7 - 10
7 - 11
-
10 min, stable
394178
7 - 9.5
-
in case of uricase entrapped in lipid vesicles, the remaining activity keeps more than 90% during the pH of 7.0-9.5, and the maximum remaining activity is 98.04% at pH 8.0 when incubated at 40°C or 40 min. For the free uricase, the maximum remaining activity is 86.59% at pH 8.5 when incubated at 40°C or 40 min
712197
7.4
-
37°C, stable for 4 days, in presence of 30 microM oxonate stable for 22 days, with exponential decrease of activity thereafter
743752
8 - 11
-
4°C, 60 days, 35% loss of activity
394132
8 - 9
-
45°C, 30 min, stable
394146
8.5 - 11
-
stable
659535
9.2
-
37°C, stable for 12 days, in presence of 30 microM oxonate stable for 22 days, with exponential decrease of activity thereafter
743752
additional information
-
uricase activity in 50 h culture broth with pH values of 5.5 and 6.0 decreases more rapidly than that in cultures with pH values of 6.5 and 7.0, at pH 5.5, about 78% of initial uricase activity is lost within 25 h, under the same conditions, more than 85% of initial uricase activity remains in culture broth of pH 6.5 and 7.0, uricase activity in 66 h culture broth with pH 7.0 degrades much more rapidly than that in samples from 50 h culture, while for pH 6.5, the uricase is still stable, loss of uricase activity is caused by the degradation in acidic environment by proteases secreted by the host cells or releases from host cell lyses, low pH may cause instability of uricase
695795