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1.7.6.1: nitrite dismutase

This is an abbreviated version!
For detailed information about nitrite dismutase, go to the full flat file.

Word Map on EC 1.7.6.1

Reaction

3 nitrite + 2 H+ = 2 nitric oxide +

nitrate
+
H2O

Synonyms

nitrophorin 4, Nitrophorin 7, NP4, NP7, Prolixin S

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.6 With a nitrogenous group as acceptor
                1.7.6.1 nitrite dismutase

Crystallization

Crystallization on EC 1.7.6.1 - nitrite dismutase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure of nitrophorin 4 at 1.5 A resolution
mutant enzyme E27V, vapor diffusion method, using 0.33% (w/v) anthrone, 0.33% (w/v) Congo Red, 0.33% (w/v) N-(2-acetamido)-2-aminoethanesulfonic acid, 0.0 2M HEPES sodium pH 6.8
protein crystals are obtained using the vapor diffusion method with the conditions containing 3.2 M ammonium phosphate (pH 7.4). X-ray crystallography of NP4 crystals soaked with nitrite reveals the formation of an eta1-N nitro complex
solid-state NMR analysis. Enzyme tends to form oligomers and precipitates at higher concentration. At concentrations of approximately 0.1-2 mM oligomers are in equilibrium with monomers. The protein oligomerizes preferably in units of trimers, hexamers, and higher oligomeric states of unidentified composition