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1.8.1.12: trypanothione-disulfide reductase

This is an abbreviated version!
For detailed information about trypanothione-disulfide reductase, go to the full flat file.

Word Map on EC 1.8.1.12

Reaction

trypanothione
+
NADP+
=
trypanothione disulfide
+
NADPH
+
H+

Synonyms

EC 1.6.4.8, LbTryR, LdTryR, Li-TryR, LiTR, N(1),N(8)-bis(glutathionyl)spermidine reductase, NADPH2:trypanothione oxidoreductase, TbTR, TCDM_11669, TcTR, TPR, TR, trypanothione disulfide reductase, trypanothione reductase, trypanothione-disulfide reductase, TryR

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.12 trypanothione-disulfide reductase

Specific Activity

Specific Activity on EC 1.8.1.12 - trypanothione-disulfide reductase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.067
-
crude extract
0.18
-
recombinant mutant C58S: transhydrogenase activity between NADPH and thio-NADP+
0.2
-
crude extract of wild-type cells
0.21
-
recombinant mutant C53A: transhydrogenase activity between NADPH and thio-NADP+
0.44
-
recombinant mutant C53S: transhydrogenase activity between NADPH and thio-NADP+
0.55
-
crude extract cells overexpressing the recombinant enzyme
1.286
-
from the partially purified extract, in 50 mM potassium phosphate containing 1 mM EDTA, 1 mM trypanothione disulfide and 0.1 mM NADPH at pH 7.5 and 25°C
112.7
-
purified enzyme
12.53
purified recombinant enzyme
137
-
recombinant purified enzyme
143.2
-
purified recombinant enzyme
2.1
-
crude extract cells overexpressing the recombinant enzyme
2.15
-
recombinant overexpressed in Trypanosoma cruzi cells
247
-
purified enzyme
272
-
purified enzyme
28.9
-
purified enzyme
6.49
-
recombinant wild-type: transhydrogenase activity between NADPH and thio-NADP+
additional information
-
several replacement clones