1.8.1.12: trypanothione-disulfide reductase
This is an abbreviated version!
For detailed information about trypanothione-disulfide reductase, go to the full flat file.
Word Map on EC 1.8.1.12
-
1.8.1.12
-
trypanosoma
-
leishmania
-
cruzi
-
chagas
-
trypanosomatids
-
leishmaniasis
-
brucei
-
trypanocidal
-
antileishmanial
-
donovani
-
promastigotes
-
trypanosomiasis
-
infantum
-
amastigotes
-
fasciculata
-
antitrypanosomal
-
crithidia
-
antiparasitic
-
flavoenzyme
-
phenothiazine
-
medicine
-
epimastigotes
-
nifurtimox
-
amazonensis
-
antiprotozoal
-
trypomastigotes
-
tryparedoxin
-
kinetoplastida
-
leishmanicidal
-
congolense
-
rhodesiense
-
miltefosine
-
antimonial
-
ts2
-
n1,n8-bisglutathionylspermidine
-
benznidazole
-
clomipramine
-
glutathionylspermidine
-
antichagasic
-
thioridazine
-
ovothiols
-
nitrofuran
-
trypanothione-dependent
-
pharmacology
- 1.8.1.12
- trypanosoma
- leishmania
- cruzi
- chagas
-
trypanosomatids
- leishmaniasis
- brucei
-
trypanocidal
-
antileishmanial
- donovani
- promastigotes
- trypanosomiasis
- infantum
- amastigotes
- fasciculata
-
antitrypanosomal
- crithidia
-
antiparasitic
-
flavoenzyme
- phenothiazine
- medicine
- epimastigotes
- nifurtimox
- amazonensis
-
antiprotozoal
- trypomastigotes
- tryparedoxin
- kinetoplastida
-
leishmanicidal
- congolense
- rhodesiense
- miltefosine
-
antimonial
- ts2
-
n1,n8-bisglutathionylspermidine
- benznidazole
- clomipramine
- glutathionylspermidine
-
antichagasic
- thioridazine
-
ovothiols
-
nitrofuran
-
trypanothione-dependent
- pharmacology
Reaction
Synonyms
EC 1.6.4.8, LbTryR, LdTryR, Li-TryR, LiTR, N(1),N(8)-bis(glutathionyl)spermidine reductase, NADPH2:trypanothione oxidoreductase, TbTR, TCDM_11669, TcTR, TPR, TR, trypanothione disulfide reductase, trypanothione reductase, trypanothione-disulfide reductase, TryR
ECTree
1.8.1.12 trypanothione-disulfide reductaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 1.8.1.12 - trypanothione-disulfide reductase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-aminobutyryl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide + NADPH + H+
2 N-(4-aminobutanoyl)-L-cysteinyl-N-[3-(dimethylamino)propyl]glycinamide + NADP+
-
substrate analogue
-
-
?
5,5'-dithiobis(N-[3-(4-methylpiperazin-1-yl)propyl]-2-nitrobenzamide) + NADPH + H+
?
-
-
-
-
r
5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) + NADPH + H+
?
-
-
-
-
?, r
ajoene + NADPH
?
-
i.e. (E,Z)-4,5,9-trithiadodeca-1,6,11-triene-9-oxide, substrate and inhibitor
-
-
?
L-beta-aspartyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide + NADPH + H+
2 L-beta-aspartyl-L-cysteinyl-N-[3-(dimethylamino)propyl]glycinamide + NADP+
-
substrate analogue
-
-
?
L-cysteinylglycyl-3-dimethylaminopropylamide disulfide + NADPH + H+
2 L-cysteinyl-N-[3-(dimethylamino)propyl]glycinamide + NADP+
-
substrate analogue
-
-
?
L-gamma-glutamyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide + NADPH + H+
2 L-gamma-glutamyl-L-cysteinyl-N-[3-(dimethylamino)propyl]glycinamide + NADP+
-
substrate analogue
-
-
?
N-benzyloxycarbonyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide + NADPH + H+
2 N-[(benzyloxy)carbonyl]-L-cysteinyl-N-[3-(dimethylamino)propyl]glycinamide + NADP+
-
substrate analogue
-
-
?
tert-butyloxycarbonyl-L-cysteinylglycyl-3-dimethylaminopropylamide disulfide + NADPH + H+
2 N-(tert-butoxycarbonyl)-L-cysteinyl-N-[3-(dimethylamino)propyl]glycinamide + NADP+
-
substrate analogue
-
-
?
trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid)
trypanothione disulfide + thionitrobenzoate
trypanothione disulfide + beta-thio-NADPH
trypanothione + beta-thio-NADP+
-
wild-type and mutant enzymes
-
-
?
trypanothione disulfide + reduced 3-acetylpyridine adenine dinucleotide
trypanothione + oxidized 3-acetylpyridine adenine dinucleotide
-
wild-type and mutant enzymes
-
-
?
trypanothione disulfide + thio-NADPH + H+
trypanothione + thio-NADP+
-
-
-
-
r
glutathionylspermidine disulfide + NADPH
2-glutathionylspermidine + NADP+
-
-
-
r
glutathionylspermidine disulfide + NADPH
2-glutathionylspermidine + NADP+
-
-
-
?
glutathionylspermidine disulfide + NADPH
2-glutathionylspermidine + NADP+
-
-
-
?
glutathionylspermidine disulfide + NADPH
2-glutathionylspermidine + NADP+
-
-
-
-
?
glutathionylspermidine disulfide + NADPH
2-glutathionylspermidine + NADP+
-
-
-
-
?
glutathionylspermidine disulfide + NADPH
2-glutathionylspermidine + NADP+
-
-
-
r
glutathionylspermidine disulfide + NADPH
2-glutathionylspermidine + NADP+
-
-
-
r
glutathionylspermidine disulfide + NADPH
2-glutathionylspermidine + NADP+
-
-
-
?
glutathionylspermidine disulfide + NADPH
2-glutathionylspermidine + NADP+
-
-
-
-
?
NADPH + trypanothione disulfide + H+
trypanothione + NADP+
-
100 microM NADPH, 105 or 93 microM trypanothione disulfide, pH 7.5, 25°C
-
-
?
NADPH + trypanothione disulfide + H+
trypanothione + NADP+
-
100 microM NADPH, 105 or 93 microM trypanothione disulfide, pH 7.5, 25°C
-
-
?
trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid)
trypanothione disulfide + thionitrobenzoate
-
-
-
-
?
trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid)
trypanothione disulfide + thionitrobenzoate
-
inhibitors are tested using the recombinant enzyme
-
-
?
trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid)
trypanothione disulfide + thionitrobenzoate
inhibitors are tested using the recombinant enzyme
-
-
?
trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid)
trypanothione disulfide + thionitrobenzoate
-
-
-
-
?
trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid)
trypanothione disulfide + thionitrobenzoate
-
-
-
?
trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid)
trypanothione disulfide + thionitrobenzoate
-
novel inhibitors are identified
-
-
?
trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid)
trypanothione disulfide + thionitrobenzoate
-
-
-
-
?
trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid)
trypanothione disulfide + thionitrobenzoate
-
-
-
-
?
trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid)
trypanothione disulfide + thionitrobenzoate
-
-
-
-
?
trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid)
trypanothione disulfide + thionitrobenzoate
-
inhibitors are tested using the recombinant enzyme
-
-
?
trypanothione + NADP+
trypanothione disulfide + NADPH + H+
-
-
-
-
?
trypanothione + NADP+
trypanothione disulfide + NADPH + H+
-
parasite soluble fraction
-
-
?
trypanothione + NADP+
trypanothione disulfide + NADPH + H+
parasite soluble fraction
-
-
?
trypanothione + NADP+
trypanothione disulfide + NADPH + H+
-
using the recombinant enzyme
-
-
?
trypanothione + NADP+
trypanothione disulfide + NADPH + H+
-
-
-
-
?
trypanothione + NADP+
trypanothione disulfide + NADPH + H+
-
-
-
-
?
trypanothione + NADP+
trypanothione disulfide + NADPH + H+
-
high-throughput screening campaign of a library of 100000 lead-like compounds identified 2-iminobenzimidazoles as a novel class of trypanothione reductase inhibitors
-
-
?
trypanothione + NADP+
trypanothione disulfide + NADPH + H+
-
using the recombinant enzyme
-
-
?
trypanothione + NADP+
trypanothione disulfide + NADPH + H+
-
-
-
-
?
trypanothione + NADP+
trypanothione disulfide + NADPH + H+
-
theoretical docking study, energy and structural output analysis is conducted on 72 nitrofuran derivatives in order to search for new and improved trypanothione reductase inhibitors
-
-
?
trypanothione + NADP+
trypanothione disulfide + NADPH + H+
-
using the recombinant enzyme
-
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
highly specific for
-
r
trypanothione disulfide + NADPH
trypanothione + NADP+
-
trypanothione is the oxidized form of N1,N8-bis-(glutathionyl)spermidine, the latter form a stable adduct with melarsen oxide
-
r
trypanothione disulfide + NADPH
trypanothione + NADP+
-
protection against oxidative stress
-
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
recombinant glutathione-trypanothione reductase-like enzyme
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
trypanothione is the oxidized form of N1,N8-bis-(glutathionyl)spermidine, the latter form a stable adduct with melarsen oxide
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
scheme for trypanothione-dependent H2O2 metabolism
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
metabolizes H2O2, recombinant and wild-type cells
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
trypanothione is the oxidized form of N1,N8-bis-(glutathionyl)spermidine, the latter form a stable adduct with melarsen oxide
-
r
trypanothione disulfide + NADPH
trypanothione + NADP+
-
physiological function is to protect the organism from oxidative stress and to assist in maintaining low intracellular levels of hydrogen peroxide
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
highly specific for
-
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
kex enzyme in the tryanothione-based redox metabolism of pathogenic trypanosomes
-
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
i.e. bisglutathionylspermidine
-
-
?
trypanothione disulfide + NADPH
trypanothione + NADP+
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
ir
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
ir
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
highly specific for trypanothione disulfide
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
-
?
trypanothione disulfide + NADPH + H+
trypanothione + NADP+
-
-
-
?
additional information
?
-
-
naphthoquinones and nitrofuran derivatives are reduced, inhibition of the enzyme
-
-
?
additional information
?
-
-
reduces several trypanothione derivatives, e.g. N1-monoglutathionylspermidine, mixed disulfide of N1-monoglutathionylspermidine and glutathione
-
-
?
additional information
?
-
-
glutaryl-L-cysteinylglycyl-3-methylaminopropylamide disulfide is no substrate
-
-
?
additional information
?
-
-
enzyme is associated with infective capacity of the parasite
-
-
?
additional information
?
-
no activity with oxidized glutathione of the recombinant enzyme
-
-
?
additional information
?
-
-
no activity with oxidized glutathione of the recombinant enzyme
-
-
?
additional information
?
-
no activity with oxidized glutathione of the recombinant enzyme
-
-
?
additional information
?
-
the rate of reduction of 0.05 mM glutathione disulfide is 0.03% of that obtained with 0.05 mM trypanothione disulfide
-
-
?
additional information
?
-
-
the rate of reduction of 0.05 mM glutathione disulfide is 0.03% of that obtained with 0.05 mM trypanothione disulfide
-
-
?
additional information
?
-
-
naphthoquinones and nitrofuran derivatives are reduced, inhibition of the enzyme
-
-
?
additional information
?
-
serve as subversive substrates in redox cycling activity: 3,3'-[polyaminobis(carbonylalkyl)]bis(1,4-naphthoquinone) derivatives, inhibitory and substrate activity depending on spacer length
-
-
?
additional information
?
-
-
serve as subversive substrates in redox cycling activity: 3,3'-[polyaminobis(carbonylalkyl)]bis(1,4-naphthoquinone) derivatives, inhibitory and substrate activity depending on spacer length
-
-
?
additional information
?
-
-
chinifur increases the oxidation of the enzyme 40fold
-
-
?
additional information
?
-
-
wild-type enzyme shows transhydrogenase activity between NADPH and thio-NADP+
-
-
?
additional information
?
-
-
reduces 5,5'-dithiobis(2-nitrobenzamides)
-
-
?
additional information
?
-
-
recombinant mutants C53S, C53A, C58S: no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+
-
-
?
additional information
?
-
-
reduces inhibitory nitrofurans, e.g. chinifur, nifuroxamide, nifuroxime and nifurprazine, and naphthoqinone derivatives
-
-
?
additional information
?
-
-
enzyme is required for maintaining the reducing intracellular environment
-
-
?
additional information
?
-
-
enzyme is required for maintaining the reducing intracellular redox equilibrium, trypanosomes lacking the enzyme are avirulent and show increased sensitivity to oxidative stress
-
-
?
additional information
?
-
-
wild-type enzyme shows transhydrogenase activity between NADPH and thio-NADP+
-
-
?
additional information
?
-
-
recombinant mutants C53S, C53A, C58S: no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+
-
-
?
