1.8.1.2: assimilatory sulfite reductase (NADPH)
This is an abbreviated version!
For detailed information about assimilatory sulfite reductase (NADPH), go to the full flat file.
Word Map on EC 1.8.1.2
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1.8.1.2
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sulfur
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siroheme
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dissimilatory
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hemoprotein
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desulfovibrio
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sulfate-reducing
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thiosulfate
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six-electron
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desulfoviridin
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o-acetylserine
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sulfhydrylase
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dsrab
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sulfurylase
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5'-phosphosulfate
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hildenborough
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ferredoxin-dependent
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o-acetyl-l-serine
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exchange-coupled
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desulfotomaculum
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brewing
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nutrition
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medicine
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synthesis
- 1.8.1.2
- sulfur
- siroheme
-
dissimilatory
- hemoprotein
- desulfovibrio
-
sulfate-reducing
- thiosulfate
-
six-electron
-
desulfoviridin
- o-acetylserine
-
sulfhydrylase
- dsrab
-
sulfurylase
- 5'-phosphosulfate
- hildenborough
-
ferredoxin-dependent
- o-acetyl-l-serine
-
exchange-coupled
- desulfotomaculum
- brewing
- nutrition
- medicine
- synthesis
Reaction
+ 3 NADP+ + 3 H2O = + 3 NADPH + 3 H+
Synonyms
CNG03990, coenzyme F420-dependent sulfite reductase, CysI, CysIJ, desulforubidin, EC 1.8.99.1, Fsr, H2S-NADP oxidoreductase, MET10, NADPH-dependent assimilatory sulfite reductase, NADPH-dependent sulfite reductase, NADPH-sulfite reductase, reductase, sulfite (reduced nicotinamide adenine dinucleotide phosphate), SIR, SIR-FP, SIR-HP, SiR/SiRHP, SIRHP, sulfite reductase, sulfite reductase hemo-subunit
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Subunits
Subunits on EC 1.8.1.2 - assimilatory sulfite reductase (NADPH)
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dimer
dodecamer
heterodimer
heterotetramer
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two alpha- and two beta-subunits, encoded by the MET10 and MET5 gene, respectively
hexamer
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alpha2,beta2,gamma2, 2 * 50000 + 2 * 42000 + 2 * 12500, SDS-PAGE
multimer
octamer of alpha subunit SiRFP, plus 4 * * 64000, beta-subunit SiRHP, calculated
additional information
dodecamer
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alpha8,beta4, 8 * 58000-60000 + 4 * 54000-57000, sedimentation under denaturing conditions
dodecamer
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NADPH-dependent assimilatory sulfite reductase (SiR) is composed of two subunits. One is a multidomain flavin binding reductase (SiRFP) and the other an iron-containing oxidase (SiRHP). Tight binding between SiRFP and SiRHP is incompatible with the electron transfer-competent interface, suggesting that the dodecameric holoenzyme consists of four SiRFP/SiRHP heterodimers and four free SiRFP molecules. Six electrons come two at a time from three NADPH cofactors to complete catalysis, which means that SiRHP must interact with SiRFP three times for every SO3(2-) that binds
dodecamer
the dodecameric complex of flavoprotein reductase subunits (SiRFP) and hemoprotein oxidase subunits (SiRHP). Small-angle neutron scattering solution structures of the interaction of SiRFP show, how both SiRHP binding to, and reduction of, SiRFP positions SiRFP for electron transfer between the subunits
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dissociation of enzyme by 5 m urea into flavoprotein octamer and hemoprotein monomers
additional information
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dissociation of enzyme by 5 m urea into flavoprotein octamer and hemoprotein monomers
additional information
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alpha chain is composed of 2 distinct domains, one binding FAD and the other binding FMN, the FMN binding domains cooperate for a head-to-head subunit interaction