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1.8.1.4: dihydrolipoyl dehydrogenase

This is an abbreviated version!
For detailed information about dihydrolipoyl dehydrogenase, go to the full flat file.

Word Map on EC 1.8.1.4

Reaction

protein N6-(dihydrolipoyl)lysine
+
NAD+
=
protein N6-(lipoyl)lysine
 +
NADH
 +
H+

Synonyms

apicoplast E3, CDS, CIP50, coronin-interacting protein, dehydrogenase, lipoamide, dehydrolipoate dehydrogenase, DHLDH, DHLipDH, diaphorase, dihydrolipoamide dehydrogenase, dihydrolipoamide dehydrogenase E3, dihydrolipoamide:NAD+ oxidoreductase, dihydrolipoic dehydrogenase, dihydrolipomide dehydrogenase, dihydrolipoyl dehydrogenase, DLD, DLD1, Dld2, DLDH, DLDH dehydrogenase, DLDH diaphorase, DLDH2, DT-diaphorase, E3, E3 component, E3 component of 2-oxoglutarate dehydrogenase complex, E3 component of acetoin cleaving system, E3 component of alpha keto acid dehydrogenase complexes, E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes, E3 component of pyruvate complex, E3 lipoamide dehydrogenase, E3 protein component of 2-oxoacid dehydrogenase multienzyme complexes, E3 subunit of the alpha-ketoglutarate dehydrogenase complex, EC 1.6.4.3, Glycine cleavage system L protein, Glycine oxidation system L-factor, hDLDH, hE3, hLADH, L-protein, LAD, LADH, LDH, LDP-Glc, LDP-Val, LipDH, lipoamide dehydrogenase, lipoamide dehydrogenase (NADH), lipoamide dehydrogenase C, lipoamide dehydrogenase2, lipoamide oxidoreductase (NADH), lipoamide reductase, lipoamide-dehydrogenase-valine, lipoate dehydrogenase, lipoic acid dehydrogenase, lipoyl dehydrogenase, LPD, LPD-GLC, LPD-VAL, LPD1, LPD2, Lpd3, LpdA, LpdC, LpdG, LpdV, More, mtLPD2, NAD(P)H:lipoamide oxidoreductase, NADH dehydrogenase, NADH diaphorase, NADH:lipoamide oxidoreductase, nicotinamide adenine dinucleotide diaphorase, ORF-E3, pdhD, pdhL, PfaE3, rhDLDH, TAase, ubiquinone reductase

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.4 dihydrolipoyl dehydrogenase

Specific Activity

Specific Activity on EC 1.8.1.4 - dihydrolipoyl dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.002
-
purified enzyme in absence of Zn2+
0.05
-
purified enzyme in presence of Zn2+
0.17
substrate 5,5'-dithiobis-(2-nitrobenzoic acid), 30°C, pH 6.5
0.19
-
substrate 5,5'-dithiobis-(2-nitrobenzoic acid), 30°C, pH 6.5
0.344
cell lysate, in 50 mM potassium phosphate buffer, pH 6.7, at 37°C
0.36
purified DLDH
0.5
-
metmyoglobin reduction, in absence of methylene blue, pH 7.5
0.6
-
O2 reduction, pH 6.0
0.8
-
cytochrome c reduction, in absence of methylene blue, pH 7.5
1.7
-
lipoic acid reduction, pH 6.0
100
substrate alpha-lipoamide, 30°C, pH 6.5
110
-
substrate alpha-lipoamide, 30°C, pH 6.5
143.1
mutant N286Q
154
-
lipoamide reduction, pH 6.0
16.2
pH 7.0, 30°C, mutant enzyme E423D, assay in the presence of 2 M KCI
16.6
-
purified recombinant mutant D473L
179.6
mutant N286D
19.1
pH 7.0, 30°C, wild-type enzyme, assay in the presence of 2 M KCI
2.5
crude extract, in the presence of 0.2 mM NAD+, in 50 mM potassium phosphate buffer (pH 6.0), at 25°C
210
-
nitric oxide reduction, pH 6.0
22.4
-
purified enzyme, at pH 6.5 and 25°C
24
pH 8.0, 37°C
280
-
purified recombinant mutant T44V enzyme
283.1
mutant D320N
295
-
mitochondrial enzyme
3.6
pH 7.0, 30°C, mutant enzyme E423Q, assay in the absence of KCI
4.5
-
metmyoglobin reduction, in presence of methylene blue, pH 7.5
4.6
pH 7.0, 30°C, mutant enzyme E423A, assay in the absence of KCI
4.7
substrate alpha-lipoic acid, 30°C, pH 6.5
4.9
pH 7.0, 30°C, mutant enzyme E423D, assay in the absence of KCI
40
Azotobacter agilis
-
-
400
-
about, purified recombinant enzyme
5
pH 7.0, 30°C, mutant enzyme E423S, assay in the absence of KCI
5.6
pH 7.0, 30°C, wild-type enzyme, assay in the absence of KCI
5.8
pH 7.0, 30°C, mutant enzyme E423S, assay in the presence of 2 M KCI
586.5
wild-type
59.2
after 23.9fold purification, in the presence of 0.2 mM NAD+, in 50 mM potassium phosphate buffer (pH 6.0), at 25°C
6.2
pH 7.0, 30°C, mutant enzyme E423A, assay in the presence of 2 M KCI
6.65
19.3fold purified enzyme, in 50 mM potassium phosphate buffer, pH 6.7, at 37°C
62
-
enzyme from chloroplast
7.2
-
2,6-dichlorophenolindophenol reduction, pH 6.0
7.8
-
substrate alpha-lipoic acid, 30°C, pH 6.5
83.06
lipoamide reduction
90.2
-
-
additional information