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1.8.1.B1: thioredoxin glutathione reductase

This is an abbreviated version!
For detailed information about thioredoxin glutathione reductase, go to the full flat file.

Word Map on EC 1.8.1.B1

Reaction

glutathione disulfide
+
NADPH
 +
H+
= 2 glutathione +
NADP+

Synonyms

cTGR, DmTrxR, EgTGR, mTGR, SmTGR, TGR, TGRsec, thioredoxin glutathione reductase, thioredoxin-glutathione reductase, thioredoxin/glutathione reductase

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.B1 thioredoxin glutathione reductase

Specific Activity

Specific Activity on EC 1.8.1.B1 - thioredoxin glutathione reductase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0125
substrate: glutathione disulfide, recombinant wild-type enzyme, pH 6.9, 25°C
0.0599
substrate: thioredoxin disulfide, mutant enzyme U642C,pH 7.4, 25°C
0.0947
substrate: thioredoxin disulfide, recombinant wild-type enzyme, pH 7.4, 25°C
10.3
-
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
10100
-
mitochondrial isoform, substrate 5,5'-dithiobis(2-nitrobenzoic acid), pH 7.8, temperature not specified in the publication
1091
-
after 31fold purification, using 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, at pH 7.8 and 25°C
11.7
-
crude extract, using GSSG as substrate, at pH 7.8 and 25°C
12.1
-
with HED as substrate, at pH 7.4 and 25°C
128.5
-
crude extract, using hydroxyethyl disulfide as substrate, at pH 7.8 and 25°C
1300
-
mitochondrial isoform, substrate glutathione disulfide, pH 7.8, temperature not specified in the publication
17.5
-
thioredoxin disulfide + NADPH
2.19
-
with GSSG as substrate, at pH 7.4 and 25°C
2140
-
cytosolic isoform, substrate 5,5'-dithiobis(2-nitrobenzoic acid), pH 7.8, temperature not specified in the publication
2243
-
after 31fold purification, using hydroxyethyl disulfide as substrate, at pH 7.8 and 25°C
229
-
after 31fold purification, using GSSG as substrate, at pH 7.8 and 25°C
3.33
-
with thioredoxin disulfide as substrate, at pH 7.4 and 25°C
35
-
crude extract, using 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, at pH 7.8 and 25°C
4.7
-
GSSG + NADPH
8.11
-
with 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, at pH 7.4 and 25°C
980
-
cytosolic isoform, substrate glutathione disulfide, pH 7.8, temperature not specified in the publication
additional information
-
1536-well based kinetic absorbance assay for thioredoxin glutathione reductase. The assay uses the catalytic reduction of DTNB by NADPH in the presence of thioredoxin glutathione reductase