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1.8.2.2: thiosulfate dehydrogenase

This is an abbreviated version!
For detailed information about thiosulfate dehydrogenase, go to the full flat file.

Word Map on EC 1.8.2.2

Reaction

2 thiosulfate +

2 ferricytochrome c
=
tetrathionate
 + 2 ferrocytochrome c + 4 H+

Synonyms

AFE_0042, Alvin_0091, AvTsdA, C8J_0815, D0Y83_01395, di-heme TsdA, DIE28_04650, diheme cytochrome c TsdA, enzymes, thiosulfate-oxidizing, MpTsdBA, oxidase, thiosulfate, Tat pathway signal sequence domain protein, tetrathionate reductase, tetrathionate synthase, thiosulfate dehydrogenase, thiosulfate oxidase, thiosulfate-acceptor oxidoreductase, thiosulfate-oxidizing enzyme, TSD, TsdA

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.2 With a cytochrome as acceptor
                1.8.2.2 thiosulfate dehydrogenase

Storage Stability

Storage Stability on EC 1.8.2.2 - thiosulfate dehydrogenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 36% loss of activity after 9 days
-
-20°C, crude extract, 50% loss of activity after 2 months
heterotrophic bacterium
-
-20°C, little loss of activity after 2 months
-
-20°C, the ability of the enzyme to couple with cytochrome c decreases during storage
-
-20°C, the activity of cell-free extracts is stable for several days in the presence of 20% v/v glycerol, without glycerol most of the activity is lost after 2 days
-
-20°C, the activity of whole cells is stable for 3 months
-
-25°C, 0.02 M phosphate, 40% loss of activity after 2 months
-
20-30% loss of activity after freezing at -20°C and thawing
-
4°C, 10% loss of activity after 6 h and 50% loss of activity after 5 days
-
4°C, 50% loss of activity after 3 days, 75% loss of activity after 14 days
-
4°C, cell-free extracts lose most of the activity after 2 days even in the presence of glycerol
-
4°C, purified enzyme, 10% loss of activity after 24 h, completely inactive after 7 days
-
4°C, stable, 2 days
-
diluted enzyme loses 10-15% of the activity each time it is frozen and thawed
-
frozen, 0.05 M phosphate buffer, pH 6.2, stable for at least 90 days
-
frozen, stable for at least 3 months
-
frozen, stable, indefinitely as measured by ferricyanide reduction
-
the activity in cell-free extracts is lost very rapidly after freezing and thawing
-