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0.00043 - 0.107
cytochrome c
additional information
additional information
-
0.00043
cytochrome c
-
mutant enzyme G473A, pH 8.5
0.00082
cytochrome c
-
mutant enzyme G473A, pH 7.0
0.00123
cytochrome c
-
mutant enzyme G473A, pH 6.0
0.00141
cytochrome c
-
wild type enzyme, pH 7.0
0.00173
cytochrome c
-
wild type enzyme, pH 6.0
0.00371
cytochrome c
-
mutant enzyme G473D, pH 8.6
0.0044
cytochrome c
-
wild type enzyme, pH 8.5
0.00474
cytochrome c
-
mutant enzyme G473D, pH 8.0
0.00536
cytochrome c
-
mutant enzyme G473W, pH 8.5
0.014
cytochrome c
-
mutant enzyme G473D, pH 7.5
0.0357
cytochrome c
-
mutant enzyme G473W, pH 7.0
0.0362
cytochrome c
-
mutant enzyme G473D, pH 7.0
0.0635
cytochrome c
-
mutant enzyme G473D, pH 6.5
0.0905
cytochrome c
-
mutant enzyme G473W, pH 6.0
0.107
cytochrome c
-
mutant enzyme G473D, pH 6.0
0.015
O2
-
-
0.00046
sulfite
-
mutant V474M, 25°C, pH 6.0
0.00129
sulfite
-
25°C, pH 6.0, 20 mM buffer, wild-type enzyme
0.00129
sulfite
-
wild type enzyme in 20 mM Tris at pH 6.0
0.00129
sulfite
-
wild type enzyme, pH 6.0
0.0013
sulfite
-
wild-type, 25°C, pH 6.0
0.00133
sulfite
wild-type, 25°C, pH 7.0
0.00134
sulfite
-
mutant V474M, 25°C, pH 7.0
0.00162
sulfite
-
25°C, pH 6.5, 20 mM buffer, wild-type enzyme
0.00162
sulfite
-
wild type enzyme, pH 6.5
0.0017
sulfite
-
mutant Y83A, pH 8.0, 25°C
0.0019
sulfite
-
mutant R472M, 25°C, pH 6.0
0.0023
sulfite
-
mutant R472Q, 25°C, pH 6.0
0.0025
sulfite
-
mutant R472M, 25°C, pH 7.0
0.0027
sulfite
-
wild-type, 25°C, pH 7.0
0.00272
sulfite
-
25°C, pH 7.0, 20 mM buffer, wild-type enzyme
0.00272
sulfite
-
wild type enzyme, pH 7.0
0.0028
sulfite
-
mutant H90F, pH 8.0, 25°C
0.00311
sulfite
-
25°C, pH 6.0, 20 mM buffer, mutant enzyme Y343F
0.0032
sulfite
-
mutant R472K, pH 7.6, 25°C
0.0032
sulfite
-
mutant V474M, 25°C, pH 8.0
0.00339
sulfite
-
wild type enzyme, pH 7.5
0.00339
sulfite
-
25°C, pH 7.5, 20 mM buffer, wild-type enzyme
0.0035
sulfite
-
mutant R472Q, 25°C, pH 7.0
0.00352
sulfite
-
25°C, pH 7.0, 100 mM buffer, mutant enzyme Y343F
0.00362
sulfite
-
25°C, pH 7.0, 100 mM buffer, wild-type enzyme
0.00367
sulfite
-
25°C, pH 7.5, 100 mM buffer, wild-type enzyme
0.0038
sulfite
-
mutant F79A, pH 8.0, 25°C
0.0039
sulfite
-
mutant H90Y, pH 8.0, 25°C
0.004
sulfite
with ferricanide, pH 7.1, 25°C, recombinant wild-type enzyme
0.00414
sulfite
-
25°C, pH 6.5, 20 mM buffer, mutant enzyme Y343F
0.0042
sulfite
with ferricanide, pH 7.1, 25°C, recombinant wild-type enzyme
0.00423
sulfite
-
25°C, pH 7.5, 100 mM buffer, mutant enzyme Y343F
0.0043
sulfite
-
wild-type, 25°C, pH 8.0
0.00435
sulfite
-
25°C, pH 8.0, 20 mM buffer, wild-type enzyme
0.00435
sulfite
-
wild type enzyme, pH 8.0
0.00453
sulfite
-
mutant enzyme G473A in 20 mM Tris at pH 6.0
0.00453
sulfite
-
mutant enzyme G473A, pH 6.0
0.00459
sulfite
-
25°C, pH 7.0, 20 mM buffer, mutant enzyme Y343F
0.0047
sulfite
-
mutant R472M, 25°C, pH 8.0
0.0048
sulfite
with ferricanide, pH 7.1, 25°C, recombinant mutant C242S/C253S/C260S/C451S
0.00503
sulfite
-
25°C, pH 8.25, 20 mM buffer, wild-type enzyme
0.00536
sulfite
-
mutant enzyme G473A, pH 7.0
0.00612
sulfite
-
25°C, pH 8.0, 100 mM buffer, wild-type enzyme
0.0062
sulfite
with ferricanide, pH 7.1, 25°C, recombinant mutant C242S/C253S/C260S/C451S
0.00635
sulfite
-
25°C, pH 7.5, 20 mM buffer, mutant enzyme Y343F
0.00728
sulfite
-
25°C, pH 8.25, 100 mM buffer, wild-type enzyme
0.0079
sulfite
with ferricanide, pH 7.1, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
0.008
sulfite
-
mutant F57Y, pH 8.0, 25°C
0.008
sulfite
wild-type, 25°C, pH 8.5
0.0082
sulfite
with ferricanide, pH 7.1, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
0.00825
sulfite
-
wild type enzyme, pH 8.5
0.00825
sulfite
-
25°C, pH 8.5, 20 mM buffer, wild-type enzyme
0.00825
sulfite
-
wild type enzyme in 20 mM Tris at pH 8.5
0.0083
sulfite
-
wild-type, 25°C, pH 8.5
0.00851
sulfite
-
25°C, pH 10.0, 20 mM buffer, mutant enzyme Y343F
0.00859
sulfite
-
25°C, pH 8.0, 20 mM buffer, mutant enzyme Y343F
0.00908
sulfite
-
25°C, pH 8.25, 20 mM buffer, mutant enzyme Y343F
0.00924
sulfite
-
25°C, pH 8.5, 20 mM buffer, mutant enzyme Y343F
0.00947
sulfite
-
25°C, pH 9.0, 20 mM buffer, mutant enzyme Y343F
0.00959
sulfite
-
25°C, pH 8.75, 20 mM buffer, wild-type enzyme
0.0096
sulfite
with ferricanide, pH 6.0, 25°C, recombinant mutant C242S/C253S/C260S/C451S
0.00963
sulfite
-
25°C, pH 9.5, 20 mM buffer, mutant enzyme Y343F
0.00992
sulfite
-
25°C, pH 9.75, 20 mM buffer, mutant enzyme Y343F
0.0107
sulfite
with ferricanide, pH 8.4, 25°C, recombinant mutant C242S/C253S/C260S/C451S
0.011
sulfite
-
wild-type, pH 8.0, 25°C
0.011
sulfite
-
25°C, pH 8.5, 100 mM buffer, wild-type enzyme
0.012
sulfite
-
mutant D342K, pH 7.6, 25°C
0.012
sulfite
-
mutant Y83F, pH 8.0, 25°C
0.0121
sulfite
with ferricanide, pH 6.0, 25°C, recombinant wild-type enzyme
0.013
sulfite
-
mutant F57A, pH 8.0, 25°C
0.013
sulfite
-
mutant R472Q, 25°C, pH 8.0
0.0144
sulfite
-
mutant V474M, 25°C, pH 9.0
0.0146
sulfite
with ferricanide, pH 8.4, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
0.0158
sulfite
-
25°C, pH 8.0, 100 mM buffer, mutant enzyme Y343F
0.016
sulfite
-
mutant R472Q, 25°C, pH 8.5
0.0166
sulfite
with ferricanide, pH 8.4, 25°C, recombinant wild-type enzyme
0.0172
sulfite
-
mutant enzyme G473A, pH 7.4
0.0174
sulfite
with ferricanide, pH 8.9, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
0.019
sulfite
with ferricanide, pH 6.0, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
0.0196
sulfite
with ferricanide, pH 8.9, 25°C, recombinant wild-type enzyme
0.021
sulfite
-
mutant R472M, 25°C, pH 9.0
0.0214
sulfite
pH not specified in the publication, temperature not specified in the publication
0.022
sulfite
-
mutant R472Q, pH 7.6, 25°C
0.022
sulfite
-
wild-type, 25°C, pH 9.0
0.0221
sulfite
-
25°C, pH 9.0, 20 mM buffer, wild-type enzyme
0.0221
sulfite
-
wild type enzyme, pH 9.0
0.0226
sulfite
-
using ferricyanide as electron acceptor
0.023
sulfite
-
mutant R472D, pH 7.6, 25°C
0.023
sulfite
-
mutant R472D/D342K, pH 7.6, 25°C
0.026
sulfite
-
25°C, pH 9.0, 100 mM buffer, wild-type enzyme
0.0288
sulfite
with ferricanide, pH 8.9, 25°C, recombinant mutant C242S/C253S/C260S/C451S
0.0319
sulfite
-
25°C, pH 8.25, 100 mM buffer, mutant enzyme Y343F
0.0319
sulfite
-
mutant V474M, 25°C, pH 9.5
0.0338
sulfite
-
using ferricyanide as electron acceptor
0.0354
sulfite
-
mutant V474M, 25°C, pH 8.5
0.04
sulfite
-
mutant V474M, 25°C, pH 10.0
0.042
sulfite
-
mutant R472M, pH 7.6, 25°C
0.045
sulfite
-
mutant R472Q, 25°C, pH 9.0
0.0487
sulfite
-
mutant enzyme G473A, pH 8.0
0.051
sulfite
-
mutant R472D, pH 6.5, 25°C
0.0529
sulfite
-
wild-type, 25°C, pH 10.0
0.0536
sulfite
-
25°C, pH 9.5, 100 mM buffer, wild-type enzyme
0.0537
sulfite
-
mutant R472M, 25°C, pH 9.5
0.0557
sulfite
-
25°C, pH 8.5, 100 mM buffer, mutant enzyme Y343F
0.0614
sulfite
-
mutant Y343F/R472Q, 25°C, pH 6.0
0.067
sulfite
-
wild-type, 25°C, pH 9.5
0.0671
sulfite
-
25°C, pH 9.5, 20 mM buffer, wild-type enzyme
0.0671
sulfite
-
wild type enzyme, pH 9.5
0.0692
sulfite
-
mutant enzyme A208D in 20 mM Tris at pH 6.0
0.0877
sulfite
-
mutant Y343F/R472Q, 25°C, pH 7.0
0.094
sulfite
-
mutant R472M, 25°C, pH 8.5
0.0947
sulfite
-
mutant Y343N, 25°C, pH 7.0
0.0953
sulfite
-
mutant Y343N, 25°C, pH 6.0
0.0967
sulfite
-
mutant R472Q, 25°C, pH 9.5
0.0969
sulfite
-
mutant R472M, 25°C, pH 10.0
0.107
sulfite
-
mutant enzyme G473A in 20 mM Tris at pH 8.5
0.107
sulfite
-
mutant enzyme G473A, pH 8.5
0.147
sulfite
-
25°C, pH 9.0, 100 mM buffer, mutant enzyme Y343F
0.181
sulfite
-
mutant R472Q, 25°C, pH 10.0
0.2827
sulfite
-
mutant Y343F/R472Q, 25°C, pH 8.0
0.297
sulfite
-
mutant Y343N, 25°C, pH 8.0
0.33
sulfite
-
mutant enzyme G473W, pH 7.0
0.59 - 1
sulfite
-
25°C, pH 9.5, 100 mM buffer, mutant enzyme Y343F
0.623
sulfite
-
mutant enzyme G473D, pH 7.0
0.712
sulfite
-
mutant Y343F/R472Q, 25°C, pH 8.5
0.774
sulfite
-
mutant enzyme G473A, pH 9.1
0.85
sulfite
-
mutant Y343N, 25°C, pH 8.5
0.99
sulfite
-
mutant enzyme G473D, pH 6.5
1.063
sulfite
-
mutant enzyme G473D, pH 7.5
1.1
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 6.0
1.223
sulfite
-
mutant enzyme G473D, pH 8.0
1.39
sulfite
-
mutant enzyme A208D in 20 mM Tris at pH 8.5
1.42
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 7.0
1.54
sulfite
-
25°C, pH 10.0, 100 mM buffer, mutant enzyme Y343F
1.66
sulfite
-
mutant enzyme G473D in 20 mM Tris at pH 6.0
1.66
sulfite
-
mutant enzyme G473D, pH 6.0
1.91
sulfite
-
mutant enzyme G473W in 20 mM Tris at pH 6.0
1.91
sulfite
-
mutant enzyme G473W, pH 6.0
2.03
sulfite
-
mutant enzyme G473W in 20 mM Tris at pH 8.5
2.034
sulfite
-
mutant enzyme G473W, pH 8.5
2.04
sulfite
-
mutant enzyme G473D in 20 mM Tris at pH 8.5
2.04
sulfite
-
mutant enzyme G473D, pH 8.5
2.14
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 8.0
2.46
sulfite
-
mutant Y343N, 25°C, pH 9.0
3.34
sulfite
-
mutant Y343F/R472Q, 25°C, pH 9.0
3.684
sulfite
-
mutant enzyme G473A, pH 10.0
4.64
sulfite
-
mutant Y343N/R472M, 25°C, pH 7.0
8.6
sulfite
mutant Y322N/R450M, 25°C, pH 7.0
9.37
sulfite
-
mutant Y343N, 25°C, pH 9.5
10.41
sulfite
-
mutant enzyme G473W, pH 9.0
11.73
sulfite
mutant Y322N/R450M, 25°C, pH 8.5
12
sulfite
-
mutant Y343N, 25°C, pH 10.0
14
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 8.5
16.8
sulfite
-
mutant Y343N/R472M, 25°C, pH 6.0
19.28
sulfite
-
mutant Y343N/R472M, 25°C, pH 8.0
25.88
sulfite
-
mutant enzyme G473D, pH 9.1
39.9
sulfite
-
mutant Y343F/R472Q, 25°C, pH 9.5
42.99
sulfite
-
mutant Y343N/R472M, 25°C, pH 8.5
55.5
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 9.0
59.6
sulfite
-
mutant Y343F/R472Q, 25°C, pH 10.0
85.64
sulfite
-
mutant Y343N/R472M, 25°C, pH 9.0
111
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 9.5
208
sulfite
-
mutant Y343N/R472m, 25°C, pH 9.5
418
sulfite
-
mutant Y343N/R472M/V474M, 25°C, pH 10.0
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetic studies
-
additional information
additional information
-
variation of KM with pH
-
additional information
additional information
steady-state kinetics
-
additional information
additional information
-
steady-state kinetics
-
additional information
additional information
kinetics of sulfite oxidase-dependent nitrite reduction, the catalyzes single-electron transfer is similar to Michaelis-Menten kinetics
-
additional information
additional information
-
kinetics of sulfite oxidase-dependent nitrite reduction, the catalyzes single-electron transfer is similar to Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten model
-
additional information
additional information
Michaelis-Menten steady-state kinetics of wild-type and mutant enzymes. All of the mutants show decreased rates of intramolecular electron transfer (IET) but increased steady-state rates of catalysis
-
additional information
additional information
-
Michaelis-Menten steady-state kinetics of wild-type and mutant enzymes. All of the mutants show decreased rates of intramolecular electron transfer (IET) but increased steady-state rates of catalysis
-