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1.8.3.2: thiol oxidase

This is an abbreviated version!
For detailed information about thiol oxidase, go to the full flat file.

Word Map on EC 1.8.3.2

Reaction

2 R'C(R)SH +

O2
=
R'C(R)S-S(R)CR'
+
H2O2

Synonyms

Ac92, AcMNPV P33, ALR, ALRp, AtSOX, augmenter of liver regeneration, DTT-oxidase, E10R, egg white oxidase, Ero1, Ero1p, ERV/ALR sulfhydryl oxidase, Erv1, Erv1p, Erv2, ERv2p, FAD-linked sulfhydryl oxidase, FAD-linked sulfhydryl oxidase ALR, FAD-sulfhydryl oxidase, flavin adenine dinucleotide-linked sulfhydryl oxidase, flavin-dependent sulfhydryl oxidase, GmQSOX1, GmQSOX2, hepatic regenerative stimulator substance, hepatopoietin, mitochondrial FAD-linked sulfhydryl oxidase ERV1, More, neuroblastoma-derived sulfhydryl oxidase, oxidase, thiol, P33, pB119L, protein pB119L, QSCN6, QSOX, QSOx1, QSOX1b, QSOX2, QSOX3, Quiescin Q6, quiescin Q6 sulfhydryl oxidase, quiescin Q6 sulfhydryl oxidase 1, quiescin Q6/sulfhydryl oxidase, quiescin sulfhydryl oxidase, quiescin sulfhydryl oxidase 1, quiescin sulhydryl oxidase, quiescin-like flavin-dependent sulfhydryl oxidase, Quiescin-sulfhydryl oxidase, quiescin/sulfhydryl oxidase, quiescin/sulfhydryl oxidase 1b, quiescin/sulphydryl oxidase, rQSOX, sfALR, SOX, Sox1, Sox2, SOXN, sulfhydryl oxidase, sulfhydryl oxidase SOx-3, sulfhydryl oxidase, P33, sulphydryl oxidase, thiol oxidase Erv1, thiooxidase

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.3 With oxygen as acceptor
                1.8.3.2 thiol oxidase

Purification

Purification on EC 1.8.3.2 - thiol oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from egg white form a riboflavin-binding, protein-deficient strain, several steps
-
from egg white, preparation of apoprotein
-
gel filtration
-
glutathione-Sepharose 4B column chromatography
-
glutathione-Sepharose column
-
intact enzyme and proteolytically cleaved enzyme in 30 kDa and 60 kDa fragments
-
pB119L is located in the insoluble fraction, it can be solubilized by addition of arginine, and a truncated version lacking 16 residues at the carboxy terminus is a soluble protein
recobinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration
-
recombinant enzyme from Escherichia coli by heat treatment, ethanol precipitation, ion exchange chromatography, and gel filtration
recombinant enzyme from HEK and Pt-K2 cells
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel chelate chromatography
recombinant His-tagged full length enzyme and C-terminal fragment from Escherichia coli strain BL21 to homogeneity by nickel affinity chromatography
recombinant His-tagged wild-type and mutant liver enzymes from Escherichia coli strain BL21(DE3)
-
recombinant protein
recombinant soluble His-tagged GmQSOX1a and GmQSOX1b from Escherichia coli strain Rosetta-gami B (DE3) by nickel affinity chromatography and gel filtration
-
transient covalent affinity chromatography with cysteinyl-CPG-glass
-