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0.91 - 1.59
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide
0.06
dabsyl L-methionine-(S)-sulfoxide
pH 7.4, 37°C, recombinant enzyme
0.026 - 0.12
dabsyl-L-methionine (S)-S-oxide
0.058 - 0.17
dabsyl-L-methionine (S)-sulfoxide
0.28 - 0.78
dabsyl-L-methionine-(S)-S-oxide
3.8 - 84
dabsylated L-methionine (S)-sulfoxide
10
dithiothreitol
wild-type, pH 7.5, 37°C
4.5 - 4.6
glutaredoxin 2
-
0.32 - 7
L-methionine (S)-sulfoxide
3.7
L-methionine (S,R)-sulfoxide
-
MsrB activity of PILB, pH 8.0, 25°C
0.0054 - 0.39
L-methionine-(S)-S-oxide
0.0548 - 0.415
N-acetyl-L-methionine-(S)-S-oxide
0.025 - 0.508
thioredoxin
0.32 - 0.52
tryparedoxin I
-
additional information
additional information
-
-
-
0.91
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide
recombinant isozyme PMSRA3, with DTT, pH 7.5, 22°C
1.59
9-fluorenylmethyl chloroformate-labeled L-methionine-(S)-S-oxide
recombinant isozyme PMSRA4, with DTT, pH 8.0, 22°C
0.026
dabsyl-L-methionine (S)-S-oxide
isolated MrsA domain, pH 7.5, 37°C
0.045
dabsyl-L-methionine (S)-S-oxide
mutant Y163A//E165A, pH 7.5, 37°C
0.068
dabsyl-L-methionine (S)-S-oxide
mutant Y163A, pH 7.5, 37°C
0.096
dabsyl-L-methionine (S)-S-oxide
mutant E165A, pH 7.5, 37°C
0.108
dabsyl-L-methionine (S)-S-oxide
mutant C285S, pH 7.5, 37°C
0.11
dabsyl-L-methionine (S)-S-oxide
mutant D164A, pH 7.5, 37°C
0.12
dabsyl-L-methionine (S)-S-oxide
wild-type, pH 7.5, 37°C
0.058
dabsyl-L-methionine (S)-sulfoxide
pH 7.5, 37°C, mutant enzyme E339A
0.095
dabsyl-L-methionine (S)-sulfoxide
pH 7.5, 37°C, mutant enzyme E193A/D197A
0.097
dabsyl-L-methionine (S)-sulfoxide
pH 7.5, 37°C, mutant enzyme Y343F
0.1
dabsyl-L-methionine (S)-sulfoxide
pH 7.5, 37°C, mutant enzyme D197A
0.108
dabsyl-L-methionine (S)-sulfoxide
pH 7.5, 37°C, mutant enzyme E193A
0.17
dabsyl-L-methionine (S)-sulfoxide
pH 7.5, 37°C, wild-type enzyme
0.28
dabsyl-L-methionine-(S)-S-oxide
-
recombinant wild-type MsrA, with DTT, pH 7.5, 37°C
0.78
dabsyl-L-methionine-(S)-S-oxide
-
recombinant truncated MsrA DELTA(1-46), with DTT, pH 7.5, 37°C
3.8
dabsylated L-methionine (S)-sulfoxide
-
purified cysteine mutant form U16C, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C
84
dabsylated L-methionine (S)-sulfoxide
-
wild type enzyme, using dithiothreitol as cosubstrate, in 50 mM sodium phosphate, pH 7.5, at 37°C
4.5
glutaredoxin 2
wild-type, pH 7.5, 37°C
-
4.6
glutaredoxin 2
mutant C15S, pH 7.5, 37°C
-
0.32
L-methionine (S)-sulfoxide
-
recombinant enzyme. isoform MSRA, at 30°C and pH 7.5
0.52
L-methionine (S)-sulfoxide
-
recombinant enzyme. isoform MSR10, at 30°C and pH 7.5
0.6
L-methionine (S)-sulfoxide
-
pH 5.5, 25°C, MsrA, steady-state conditions
3.7
L-methionine (S)-sulfoxide
-
pH 8.0, 25°C, cofactor thioredoxin
7
L-methionine (S)-sulfoxide
-
pH 8.0, 25°C, MsrA, steady-state conditions
0.0054
L-methionine-(S)-S-oxide
30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S
0.098
L-methionine-(S)-S-oxide
30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme
0.23
L-methionine-(S)-S-oxide
pH 7.5, 30°C, cosubstrate: tryparedoxin I
0.39
L-methionine-(S)-S-oxide
30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme
0.0548
N-acetyl-L-methionine-(S)-S-oxide
30°C, pH 7.0, cosubstrate: thioredoxin, mutant enzyme C25S
0.203
N-acetyl-L-methionine-(S)-S-oxide
30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme
0.22
N-acetyl-L-methionine-(S)-S-oxide
pH 7.5, 30°C, cosubstrate: tryparedoxin I
0.415
N-acetyl-L-methionine-(S)-S-oxide
30°C, pH 7.0, cosubstrate: thioredoxin, wild-type enzyme
0.025
thioredoxin
pH 7.5, 30°C, cosubstrate: L-methionine-(S)-S-oxide
0.048
thioredoxin
-
in 20 mM Tris-HCl, pH 8.0, 30 mM KCl, 10 mM MgCl2, at 37°C
0.083
thioredoxin
30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme
0.088
thioredoxin
mutant C86S/C206S, pH 8.0, 25°C
0.1
thioredoxin
mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C
0.1
thioredoxin
mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C
0.102
thioredoxin
30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme
0.11
thioredoxin
wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C
0.13
thioredoxin
mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C
0.15
thioredoxin
wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C
0.17
thioredoxin
mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C
0.32
thioredoxin
30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide, wild-type enzyme
0.508
thioredoxin
30°C, pH 7.0, cosubstrate: L-methionine-(S)-S-oxide, wild-type enzyme
0.32
tryparedoxin I
-
recombinant enzyme. isoform MSRA, at 30°C and pH 7.5
-
0.467
tryparedoxin I
pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(S)-S-oxide
-
0.52
tryparedoxin I
-
recombinant enzyme. isoform MSR10, at 30°C and pH 7.5
-