Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.8.4.12: peptide-methionine (R)-S-oxide reductase

This is an abbreviated version!
For detailed information about peptide-methionine (R)-S-oxide reductase, go to the full flat file.

Word Map on EC 1.8.4.12

Reaction

L-methionine (R)-sulfoxide
+
thioredoxin
=
L-methionine
 +
thioredoxin disulfide
 +
H2O

Synonyms

1-Cys methionine sulfoxide reductase B, 1-Cys MSRB, CBS-1, CBS1, cysteine-containing methionine-R-sulfoxide reductase, LOC100305558, LOC100798757, methionine sulfoxide reductase, methionine sulfoxide reductase B, methionine sulfoxide reductase B1, methionine sulfoxide reductase B2, methionine sulfoxide reductase B3, methionine sulfoxide reductase B8, methionine sulfoxide reductase MsrB3, methionine sulfoxide reductases B, methionine sulfoxide reductases B2, methionine sulphoxide reductase, methionine-R-sulfoxide reductase, methionine-R-sulfoxide reductase B, methionine-R-sulfoxide reductase B2, MetO reductase, More, MSR, MsrA, MsrA/B, MsrA/MsrB, msrAB, MsrABTk, MsrB, MSRB1, MsrB2, MsrB3, MsrB3A, MSRB4, MsrB5, MsrB7, MsrB8, MsrBA, Mxr2, NtMsrB2, OsMSRB5, PaMsrB1, peptide methionine sulfoxide reductase, peptide methionine sulfoxide reductase type B, PilB, PilB protein, PMSR, Sel-X, selenocysteine-containing methionine-R-sulfoxide reductase, selenoprotein R, SelR, sulindac reductase, TbmsrB, TCDM_06423, YeaA

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.4 With a disulfide as acceptor
                1.8.4.12 peptide-methionine (R)-S-oxide reductase

Purification

Purification on EC 1.8.4.12 - peptide-methionine (R)-S-oxide reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HiTrap column chromatography and Sephadex G-25 gel filtration
-
Ni-NTA column chromatography, and gel filtration
Ni2+-affinity column chromatography
-
partial purification of native MsrB from liver, recombinant modified MsrB from Escherichia coli
-
partially by subcellular fractionation
-
partially, cell fragmentation
-
recombinant C-terminally His-tagged wild-type and mutant MsrB to homogeneity
-
recombinant enzyme from Escherichia coli
-
recombinant enzyme from Escherichia coli strain DH5alpha
-
recombinant His-tagged enzyme from Escherichia coli
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration, to homogeneity
recombinant His-tagged full length tandem enzyme, MsrA, and MsrB domains from Escherichia coli, tags are removed by thrombin digestion
recombinant His-tagged PilB forms in Escherichia coli strain Xl-1 blue by nickel affinity chromatography
-
recombinant His-tagged PilB from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography
-
recombinant MsrB
-
recombinant MsrB domain from Escherichia coli
-
recombinant MsrB from Escherichia coli strain BL21(DE3)
-
recombinant N-terminally His-tagged full-length PilB and MsrB domain variants from Escherichia coli by nickel affinity chromatography followed by cleavage of the His-Tag through thrombin, followed by gel filtration and ion exchange chomatography
-
recombinant plastidic isozyme pPMSR from Escherichia coli strain BL21(DE3)
-
recombinant plastidic isozymes MsrB1 and MsrB2 from Escherichia coli
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heparin affinity chromatography
-
recombinant wild-type and mutant isozyme MsrB3 from Escherichia coli strain BL21(DE3)
-
recombinant wild-type and mutant isozymes MsrB1 and MsrB2 from Escherichia coli strain BL21(DE3) and NIH-3T3 cells
-
Talon Co2+ affinity resin column chromatography
-