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1.1
(S)-1-nonen-4-ol
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MsrB, pH 8.0, 25°C, cofactor thioredoxin
0.035
CDSP32
-
pH 8.0, 37°C, cosubstrate: dabsyl-L-methionine (R)-sulfoxide, MSRB1 activity
-
0.004 - 0.116
dabsyl-L-methionine (R)-S-oxide
0.002 - 0.157
dabsyl-L-methionine (R)-sulfoxide
0.09
dithiothreitol
-
recombinant enzyme, in 50 mM Tris-HCl, pH 8.0, at 37°C
0.0145
glutaredoxin
30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide
0.02 - 0.48
glutaredoxin C4
-
0.03 - 0.58
glutaredoxin S12
-
0.01 - 2.86
L-methionine (R)-sulfoxide
2.8
L-methionine (R,S)-sulfoxide
-
MsrB activity of PILB, pH 8.0, 25°C
0.002 - 0.83
L-methionine-(R)-S-oxide
0.24 - 2.04
N-acetyl-L-methionine (R)-sulfoxide
0.00052 - 0.014
N-acetyl-L-methionine-(R)-S-oxide
0.0000032 - 0.0056
thioredoxin
1.28
thioredoxin h1
-
isoform MSRB2 wild type enzyme
-
0.0048
trypanothione disulfide
pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, mutant enzyme C72S/C74S
0.00067 - 0.00583
tryparedoxin I
-
additional information
additional information
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0.004
dabsyl-L-methionine (R)-S-oxide
isolated MrsB domain, pH 7.5, 37°C
0.025
dabsyl-L-methionine (R)-S-oxide
mutant Y163A//E165A, pH 7.5, 37°C
0.036
dabsyl-L-methionine (R)-S-oxide
mutant Y163A, pH 7.5, 37°C
0.068
dabsyl-L-methionine (R)-S-oxide
mutant E165A, pH 7.5, 37°C
0.083
dabsyl-L-methionine (R)-S-oxide
mutant D164A, pH 7.5, 37°C
0.102
dabsyl-L-methionine (R)-S-oxide
mutant C11S, pH 7.5, 37°C
0.116
dabsyl-L-methionine (R)-S-oxide
wild-type, pH 7.5, 37°C
0.002
dabsyl-L-methionine (R)-sulfoxide
-
isoform MSRB1 wild type enzyme, in the presence of 20 mMTris(2-carboxyethyl) phosphine hydrochloride
0.005
dabsyl-L-methionine (R)-sulfoxide
-
isoform MSRB1 mutant T132C, in the presence of 20 mM dithioerythritol
0.012
dabsyl-L-methionine (R)-sulfoxide
-
isoform MSRB1 mutant T132A, in the presence of 20 mM dithioerythritol
0.014
dabsyl-L-methionine (R)-sulfoxide
-
isoform MSRB2 wild type enzyme, in the presence of 20 mMTris(2-carboxyethyl) phosphine hydrochloride
0.028
dabsyl-L-methionine (R)-sulfoxide
-
isoform MSRB2 wild type enzyme, in the presence of 20 mM dithioerythritol
0.045
dabsyl-L-methionine (R)-sulfoxide
pH 7.5, 37°C, mutant enzyme E339A
0.047
dabsyl-L-methionine (R)-sulfoxide
-
isoform MSRB1 mutant C116S, in the presence of 20 mM dithioerythritol
0.05
dabsyl-L-methionine (R)-sulfoxide
pH 7.5, 37°C, mutant enzyme E193A/D197A
0.064
dabsyl-L-methionine (R)-sulfoxide
-
isoform MSRB2 mutant C134T, in the presence of 20 mM dithioerythritol
0.073
dabsyl-L-methionine (R)-sulfoxide
pH 7.5, 37°C, mutant enzyme D197A
0.075
dabsyl-L-methionine (R)-sulfoxide
-
isoform MSRB1 wild type enzyme, in the presence of 20 mM dithioerythritol
0.078
dabsyl-L-methionine (R)-sulfoxide
pH 7.5, 37°C, mutant enzyme E193A
0.087
dabsyl-L-methionine (R)-sulfoxide
pH 7.5, 37°C, mutant enzyme Y343F
0.157
dabsyl-L-methionine (R)-sulfoxide
pH 7.5, 37°C, wild-type enzyme
0.02
glutaredoxin C4
-
isoform MSRB2 wild type enzyme
-
0.3
glutaredoxin C4
-
isoform MSRB2 mutant enzyme C134T
-
0.34
glutaredoxin C4
-
isoform MSRB1 mutant enzyme C116S
-
0.37
glutaredoxin C4
-
isoform MSRB1 mutant enzyme T132A
-
0.48
glutaredoxin C4
-
isoform MSRB1 wild type enzyme
-
0.03
glutaredoxin S12
-
isoform MSRB2 wild type enzyme
-
0.17
glutaredoxin S12
-
pH 8.0, 37°C, cosubstrate: dabsyl-L-methionine (R)-sulfoxide, MSRB1 activity
-
0.31
glutaredoxin S12
-
isoform MSRB1 mutant enzyme T132A
-
0.36
glutaredoxin S12
-
isoform MSRB2 mutant enzyme C134T
-
0.5
glutaredoxin S12
-
isoform MSRB1 mutant enzyme C116S
-
0.58
glutaredoxin S12
-
isoform MSRB1 wild type enzyme
-
0.01
L-methionine (R)-sulfoxide
-
in vitro, substrate free L-methionine (R)-sulfoxide
0.13
L-methionine (R)-sulfoxide
-
isoform MSRB4, pH and temperature not specified in the publication
2.86
L-methionine (R)-sulfoxide
-
isoform MSRB2, pH and temperature not specified in the publication
0.002
L-methionine-(R)-S-oxide
-
recombinant wild-type cysteine-containing isozyme MsrB1 expressed in NIH 3T3 cells
0.002
L-methionine-(R)-S-oxide
pH 7.5, 30°C, cosubstrate: tryparedoxin I, wild-type enzyme
0.0076
L-methionine-(R)-S-oxide
30°C, pH 7.0, cosubstrate: glutaredoxin
0.02
L-methionine-(R)-S-oxide
-
recombinant isozyme MsrB2, with dithioerythritol
0.07
L-methionine-(R)-S-oxide
-
recombinant isozyme MsrB1, with dithioerythritol
0.22
L-methionine-(R)-S-oxide
-
recombinant wild-type selenocysteine-containing isozyme MsrB1 expressed in NIH 3T3 cells
0.46
L-methionine-(R)-S-oxide
-
recombinant wild-type isozyme MsrB2 expressed in Escherichia coli
0.83
L-methionine-(R)-S-oxide
-
recombinant wild-type isozyme MsrB3 expressed in Escherichia coli
0.24
N-acetyl-L-methionine (R)-sulfoxide
-
isoform MSRB4, pH and temperature not specified in the publication
2.04
N-acetyl-L-methionine (R)-sulfoxide
-
isoform MSRB2, pH and temperature not specified in the publication
0.00052
N-acetyl-L-methionine-(R)-S-oxide
pH 7.5, 30°C, cosubstrate: tryparedoxin I, mutant enzyme C72S
0.0016
N-acetyl-L-methionine-(R)-S-oxide
pH 7.5, 30°C, cosubstrate: tryparedoxin I, mutant enzyme C74S
0.0023
N-acetyl-L-methionine-(R)-S-oxide
pH 7.5, 30°C, cosubstrate: trypanothione, mutant enzyme C72S/C74S
0.0048
N-acetyl-L-methionine-(R)-S-oxide
pH 7.5, 30°C, cosubstrate: tryparedoxin I, wild-type enzyme
0.014
N-acetyl-L-methionine-(R)-S-oxide
30°C, pH 7.0, cosubstrate: glutaredoxin
0.0000032
thioredoxin
-
pH 8.0, 37°C
0.0043
thioredoxin
30°C, pH 7.0, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide
0.0056
thioredoxin
pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, wild-type enzyme
0.00067
tryparedoxin I
pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, mutant enzyme C72S
-
0.0025
tryparedoxin I
pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, mutant enzyme C74S
-
0.00583
tryparedoxin I
pH 7.5, 30°C, cosubstrate: N-acetyl-L-methionine-(R)-S-oxide, wild-type enzyme
-
additional information
additional information
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-
-
additional information
additional information
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reductase step kinetic parameters of the wild-type and mutated MsrB
-