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1.8.5.1: glutathione dehydrogenase (ascorbate)

This is an abbreviated version!
For detailed information about glutathione dehydrogenase (ascorbate), go to the full flat file.

Word Map on EC 1.8.5.1

Reaction

2 glutathione +

dehydroascorbate
=
glutathione disulfide
 +
ascorbate

Synonyms

At1g19570, At1g75270, At5g16710, AtDHAR, AtDHAR1, AtDHAR2, AtDHAR3, CrDHAR1, DasA reductase, dehydroascorbate reductase, dehydroascorbate reductase 2, dehydroascorbic acid reductase, dehydroascorbic reductase, dehydrogenase, glutathione (ascorbate), DHA reductase, DHA-R, DHAR, DHAR1, DHAR2, DHAR3, DHAR3a, DHAR4, DHAR5, dioscorin, GDOR, glutathione dehydroascorbate reductase, glutathione-dependent dehydroascorate reductase, glutathione:dehydroascorbic acid oxidoreductase, GRX1, GRX2, GSH-DHAR, GSH:DHA-oxidoreductase, GSTO, GSTO1, GSTO1-1, GSTO2, GSTO2-2, LcDHAR, metallothionein-1, metallothionein-2, More, MT-I, MT-II, nlGSTO, OsDHAR, OsDHAR1, PbDHAR, PgDHAR1, PtrDHAR1, PtrDHAR2, PtrDHAR3A, PtrDHAR3B, SPD1

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.5 With a quinone or similar compound as acceptor
                1.8.5.1 glutathione dehydrogenase (ascorbate)

Temperature Stability

Temperature Stability on EC 1.8.5.1 - glutathione dehydrogenase (ascorbate)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
the half-life of deactivation of the enzyme at 100°C is 8.5 min, and its thermal inactivation rate constant Kd is 0.0652/min
30 - 60
the enzyme retains more than 80% activity between 30 and 60°C for 20 min
35 - 55
-
when preincubated at 35°C for 15 min, the enzyme activity is almost unaffected but begins to decrease when the temperature sets above 40°C. Isoform DHAR1 and DHAR2 still retain 61 and 73% of their initial activity when pre-incubated at 55°C for 15 min
42
-
pH 7.0-8.0, stable up to
53
at 53°C, both GSTO2-2 isoforms show a similar loss of activity
55
the recombinant PbDHAR is a thermostable enzyme, retaining 77% of its initial activity at 55°C
60
-
10 min, complete inactivation
65
-
complete loss of activity above
75
-
5 min, 90% loss of activity