1.8.99.B2: tetrathionate reductase

This is an abbreviated version!
For detailed information about tetrathionate reductase, go to the full flat file.

Reaction

Synonyms

AFE_0042, C8J_0815, CjTsdA, di-heme TsdA, tetrathionate-forming thiosulfate dehydrogenase, tetrathionate-forming TSD, thiosulfate dehydrogenase, thiosulfate:quinone reductase, TQO, TSD, TsdA, ttrA, WsTsdA

ECTree

     1 Oxidoreductases

         1.8 Acting on a sulfur group of donors

             1.8.99 With unknown physiological acceptors

                1.8.99.B2 tetrathionate reductase

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Results	in table
2	Activating Compound
2	AA Sequence
2	Application
4	Cloned(Commentary)
11	Cofactor
11	General Information
14	Inhibitors
1	kcat/KM [mM/s]
2	KM Value [mM]
7	Localization
14	Metals/Ions
8	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
18	Organism
8	pH Optimum
1	pH Range
1	pH Stability
3	Protein Variants
7	Purification (Commentary)
1	Reaction
13	Reference
5	Source Tissue
8	Specific Activity [micromol/min/mg]
35	Substrates and Products (Substrate)
12	Subunits
32	Synonyms
8	Temperature Optimum [°C]
2	Temperature Stability [°C]
1	Turnover Number [1/s]

Purification

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Purification on EC 1.8.99.B2 - tetrathionate reductase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Campylobacter jejuni

-

726008

71fold enrichment with a recovery of 4.3% of the total activity

Acidithiobacillus thiooxidans

-

737877

native enzyme 71.4fold from the membrane fraction of strain SH by solubilization with n-dodecyl-beta-D-maltopyranoside, ultracentrifugation, anion exchange chromatography, dialysis, ultrafiltration, and gel filtration

Acidithiobacillus thiooxidans

-

742121

native enzyme 71.4fold from the solubilized membrane fraction by ultracentrifugation, anion exchange chromatography, dialysis, and ultrafiltration

Acidithiobacillus thiooxidans

-

742121

native periplasmic enzyme from strain ATCC 23270 and recombinant enzyme from Escherichia coli strain BL21(DE3) celll extract by ammonium sulfate fractionation, cation exchange chromatography, dialysis, hydrophobic interaction chromatography, ultrafilatration, and gel filtration

Acidithiobacillus ferrooxidans

B7J3E3

741675

recombinant Strep-tagged enzyme TsdA 41fold from Escherichia coli strains BL21(DE3) and C43(DE3) by membrane solubilization, affinity chromatography, desalting gel filtration, and ultrafiltration recombinant Strep-tagged TsdC by two steps of ultracentrifugation at 100000 x g, followed by affinity chromatography

Wolinella succinogenes

-

764683

using chromatography on Phenyl-Sepharose column, CM-cellulose column and hydroxylapatite column

Acidithiobacillus thiooxidans

-

394129