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1.97.1.12: photosystem I

This is an abbreviated version!
For detailed information about photosystem I, go to the full flat file.

Word Map on EC 1.97.1.12

Reaction

reduced plastocyanin
+
oxidized ferredoxin
+
hnu
=
oxidized plastocyanin
+
reduced ferredoxin

Synonyms

OsCRR6, photosystem I P700 chlorophyll a apoprotein A2, PS I, PS-I, PS-I complex, PsaB, PsaF, PSI, PSI core complex

ECTree

     1 Oxidoreductases
         1.97 Other oxidoreductases
             1.97.1 Sole sub-subclass for oxidoreductases that do not belong in the other subclasses
                1.97.1.12 photosystem I

Engineering

Engineering on EC 1.97.1.12 - photosystem I

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D612H/E613H
mutation in subunit PsaB of photosystem I. Photosystem I harboring the has a high affinity toward binding of the electron donors and possesses an altered pH dependence of electron transfer with plastocyanin and cytochrome c6. The mutant strain exhibits a strong light sensitive growth phenotype, indicating that decelerated turnover between plastocyanin/cytochrome c6 and photosystem I with respect to electron transfer is deleterious to the cells
F689N
site-directed mutagenesis of subunit PsaA, the mutation causes in an about 100fold decrease in the observed rate of cofactor phylloquinone PhQA- oxidation, resulting in a lifetime that exceeds that of the terminal electron donor, P700+. This situation allows a second photochemical charge separation event to be initiated before PhQA- has decayed, thereby mimicking in PSI a situation that occurs in type II reaction centers. Simulation of the pump-pump kinetics in PsaA-F689N, overview
N591L
site-directed mutagenesis of psaB, the mutant shows structural differences and altered activity compared to the wild-type enzyme, detailed overview
N604L
site-directed mutagenesis of psaA, the mutant shows structural differences and altered activity compared to the wild-type enzyme, detailed overview
F689N
-
site-directed mutagenesis of subunit PsaA, the mutation causes in an about 100fold decrease in the observed rate of cofactor phylloquinone PhQA- oxidation, resulting in a lifetime that exceeds that of the terminal electron donor, P700+. This situation allows a second photochemical charge separation event to be initiated before PhQA- has decayed, thereby mimicking in PSI a situation that occurs in type II reaction centers. Simulation of the pump-pump kinetics in PsaA-F689N, overview
-
F647C
-
mutant strain cannot grow under photoautotrophic conditions because of low photosystem I activity, possibly due to low levels of proteins
F649C/G650I
-
mutant strain cannot grow under photoautotrophic conditions because of low photosystem I activity, possibly due to low levels of proteins
H651C/L652M
-
mutant strain cannot grow under photoautotrophic conditions because of low photosystem I activity, possibly due to low levels of proteins
S641C/V642I
-
mutation in subunit PsaB. Mutant strain grows photoautotrophically and shows no obvious reduction in the photosystem I activity. Kinetics of P700 re-reduction by plastocyanin remains unaltered
W643C/A644I
-
mutation in subunit PsaB. Mutant strain grows photoautotrophically and shows no obvious reduction in the photosystem I activity. Kinetics of P700 re-reduction by plastocyanin remains unaltered
W645C
-
mutation in subunit PsaB. Mutant strain grows photoautotrophically and shows no obvious reduction in the photosystem I activity. Kinetics of P700 re-reduction by plastocyanin remains unaltered
N591L
site-directed mutagenesis of psaB, the mutant shows structural differences and altered activity compared to the wild-type enzyme, detailed overview
N604L
site-directed mutagenesis of psaA, the mutant shows structural differences and altered activity compared to the wild-type enzyme, detailed overview
additional information