2.1.1.113: site-specific DNA-methyltransferase (cytosine-N4-specific)

This is an abbreviated version!
For detailed information about site-specific DNA-methyltransferase (cytosine-N4-specific), go to the full flat file.

Reaction

Synonyms

BamHI [cytosine-N4] MTase, cytosine-N5-methyltransferase, DNA-(N4-cytosine)-methyltransferase, DNA[cytosine-N4]methyltransferase, DraR1, HP_1368, M.AatII, M.AvaI, M.BalI, M.BcnIA, M.BcnIB, M.BglII, M.BsoBI, M.BstNI, M.CbeI, M.CsyAIP, M.CsyBIP, M.EsaBC1I, M.EsaBC2I, M.EsaLHCI, M.Hpy99ORF244P, M.Hpy99ORF248P, M.Hpy99ORF629P, M.HpyAIIP, M.HpyAXIIBP, M.MjaIP, M.MjaV, M.MthZI, M.MwoI, M.NcoI, M.NgoMXV, M.Pac25I, M.PhiGIP, M.PhiHII, M.PhoIIIP, M.PspGI, M.PvuII, M.RsaI, M.SapIA, M.SapIB, M.ScaI, M.SfiI, M.SmaI, M.StyCIP, M.XcyI, M.XmaI, M.XveII protein, M2.MboII, M2.NcuI, m4C-forming Mtase, m4C-MTase, modification methylase, More, N4-cytosine methyltransferase, N4-cytosine MTase, N4-methyltransferase, N4mC MTase, ORF16000P, restriction-modification system, sll0729, SuaI

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.113 site-specific DNA-methyltransferase (cytosine-N4-specific)

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Results	in table
1	Activating Compound
9014	AA Sequence
5	CAS Registry Number
10	Cloned(Commentary)
3	Cofactor
2	Crystallization (Commentary)
9	General Information
4	IC50 Value
11	Inhibitors
5	KM Value [mM]
3	Metals/Ions
10	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
124	Organism
1	PDB ID
3	pH Optimum
7	Purification (Commentary)
3	Reaction
1	Reaction Type
74	Reference
1	Source Tissue
3	Specific Activity [micromol/min/mg]
78	Substrates and Products (Substrate)
7	Subunits
94	Synonyms
3	Temperature Optimum [°C]
3	Turnover Number [1/s]

Purification

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Purification on EC 2.1.1.113 - site-specific DNA-methyltransferase (cytosine-N4-specific)

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

gel filtration, recombinant protein for in vitro methyltransferase activity assay

Xanthomonas campestris

Q3BWM2

686788

native and selenomethionyl M.PvuII

Proteus vulgaris

-

485129

Ni-NTA column chromatography

Caldicellulosiruptor bescii

B9MK94

735063

purified from E. coli cells expressing the genes for M.BcnIA and M.BcnIB

Brevibacillus centrosporus

-

485135

recombinant M2.MboII 3.6fold to electrophoretic homogeneity using a four-step chromatographic procedure, involving adsorption and hydroxylapatite chromatography followed by hydroophobic interaction and heparin affinity chromatography, from Escherichia coli

Moraxella bovis

-

705556

recombinant M2.NcuI 3.6fold to electrophoretic homogeneity using a four-step chromatographic procedure, involving adsorption and hydroxylapatite chromatography followed by hydrophobic interaction and heparin affinity chromatography, from Escherichia coli

Moraxella cuniculi

-

705556

recombinant wild-type enzyme from Escherichia coli by adsorption chromatography and dialysis to homogeneity

Pyrococcus sp. GI-H

-

694446