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2.1.1.158: 7-methylxanthosine synthase

This is an abbreviated version!
For detailed information about 7-methylxanthosine synthase, go to the full flat file.

Word Map on EC 2.1.1.158

Reaction

S-adenosyl-L-methionine
+
Xanthosine
=
S-adenosyl-L-homocysteine
+
7-methylxanthosine

Synonyms

7-methylxanthine 3-N-methyltransferase, 7-methylxanthine synthase, 7-methylxanthosine synthase, 7-methylxanthosine synthase 1, 7-N-methyltransferase, 7NMT, CmXRS1, N-methyltransferase, SAM:xanthosine 7-N-methyltransferase, xanthosine methyltransferase, xanthosine-N7-methyltransferase, xanthosine:S-adenosyl-L-methionine methyltransferase, XMT, XMT1, XRS1

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.158 7-methylxanthosine synthase

Crystallization

Crystallization on EC 2.1.1.158 - 7-methylxanthosine synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant wild-type and selenomethionine-labeled XMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 1 mM S-adenosyl-L-cysteine, and 1 mM xanthosine, 1-3 days, 20°C, X-ray diffraction structure determination and analysis at 2.8-3.0 A resolution
purified recombinant wild-type and selenomethionine-labeled XMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 2 mM S-adenosyl-L-cysteine, and 2 mM xanthosine, 1-3 days, 20°C, plate-like crystals, X-ray diffraction structure determination and analysis at 2.2 A resolution
using Linbro plates and the conventional hanging-drop technique, in the presence of the demethylated cofactor S-adenosyl-l-cysteine or xanthosine