2.1.1.158: 7-methylxanthosine synthase

This is an abbreviated version!
For detailed information about 7-methylxanthosine synthase, go to the full flat file.

Reaction

Synonyms

7-methylxanthine 3-N-methyltransferase, 7-methylxanthine synthase, 7-methylxanthosine synthase, 7-methylxanthosine synthase 1, 7-N-methyltransferase, 7NMT, CmXRS1, N-methyltransferase, SAM:xanthosine 7-N-methyltransferase, xanthosine methyltransferase, xanthosine-N7-methyltransferase, xanthosine:S-adenosyl-L-methionine methyltransferase, XMT, XMT1, XRS1

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.158 7-methylxanthosine synthase

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Results	in table
7	AA Sequence
1	Application
1	CAS Registry Number
11	Cloned(Commentary)
1	Cofactor
3	Crystallization (Commentary)
1	Expression
2	General Information
2	KM Value [mM]
1	Localization
3	Metals/Ions
9	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
20	Organism
6	Pathway
6	pH Optimum
2	pI Value
19	Protein Variants
5	Purification (Commentary)
3	Reaction
20	Reference
35	Source Tissue
3	Specific Activity [micromol/min/mg]
34	Substrates and Products (Substrate)
6	Subunits
31	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]

Engineering

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Engineering on EC 2.1.1.158 - 7-methylxanthosine synthase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

A23S

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity

721124

A23S/L191P/H219R

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity

721124

A23S/P104Q/H219R

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity

721124

A23S/P104Q/Q161H/H219R

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows 60% of wild-type 7-methylation activity

721124

A23S/P104Q/Q161H/L191P/H219R

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity

721124

A23S/Q161H/H219R

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity

721124

A23S/Q161H/L191P/H219R

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity

721124

H219R

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity

721124

L191P

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity

721124

P104Q

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity

721124

P104Q/L191P

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity

721124

P104Q/Q161H

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity

721124

P104Q/Q161H/L191P

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity

721124

Q161H

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity

721124

Q161H/L191P

Coffea sp.

-

site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity

721124

additional information

5 entries

additional information

Coffea arabica

Q9AVK0

reduction of the second enzyme of the pathway, 7-methylxanthine methyltransferase, EC 2.1.1.159, leads to reduced XMT1 expression

670565

additional information

Coffea arabica

-

reduction of the second enzyme of the pathway, 7-methylxanthine methyltransferase, EC 2.1.1.159, leads to reduced XMT1 expression

670565

additional information

Coffea arabica

Q9AVK0

transgenic Nicotiana tabacum plant leaves expressing all three enzymes required for the biosynthesis of caffeine are no longer eaten by the tobacco cutworm caterpillars, Spodoptera litura, overview

670568

additional information

Coffea canephora

-

reduction of the second enzyme of the pathway, 7-methylxanthine methyltransferase, EC 2.1.1.159, leads to reduced XMT1 expression

670565

additional information

Coffea sp.

-

construction of several enzyme mutants, overview. The mutants of CmXRS1, that have 3-N methylation activity and produce caffeine from paraxanthine as a substrate, need to have replacement of the glutamine residue by histidine at position 161 in the CmXRS1 sequence, i.e. a Q161H mutation, overview

721124