2.1.1.160: caffeine synthase
This is an abbreviated version!
For detailed information about caffeine synthase, go to the full flat file.
Word Map on EC 2.1.1.160
Reaction
Synonyms
1-N-methyltransferase, 1NMT, 3, 7-dimethylxanthine methyltransferase, 3,7-dimethylxanthine methyltransferase, 7-N-methylxanthine methyltransferase, caffeine synthase, caffeine synthase 1, CCS, CCS1, coffee caffeine synthase 1, CS, CtCS6, DXMT, DXMT1, N-1 methyltransferase, TCS, TCS1, tea caffeine synthase, tea caffeine synthase 1, theobromine 1-N-methyltransferase
ECTree
2.1.1.160 caffeine synthaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.1.1.160 - caffeine synthase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 1,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
S-adenosyl-L-methionine + 1-methylxanthine
S-adenosyl-L-homocysteine + 1,3-dimethylxanthine
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
S-adenosyl-L-methionine + 3-methylxanthine
S-adenosyl-L-homocysteine + 1,3-dimethylxanthine
S-adenosyl-L-methionine + 7-methylxanthine
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
S-adenosyl-L-methionine + 1,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
i.e. paraxanthine
i.e. caffeine
-
?
S-adenosyl-L-methionine + 1,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
i.e. paraxanthine, alternative route in caffeine biosynthesis
i.e. caffeine
-
?
S-adenosyl-L-methionine + 1,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
i.e. paraxanthine
i.e. caffeine
-
?
S-adenosyl-L-methionine + 1,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
i.e. paraxanthine, alternative route in caffeine biosynthesis
i.e. caffeine
-
?
S-adenosyl-L-methionine + 1,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
northern blot analysis and in situ hybridisation reveals a higher expression of caffeine synthase in younger leaves, in summer, and in leaves of the shaded and fertilized plants
-
-
?
S-adenosyl-L-methionine + 1,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
-
-
?
S-adenosyl-L-methionine + 1,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
Coffea sp.
-
-
-
-
?
S-adenosyl-L-methionine + 1,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
Coffea sp.
-
paraxanthine is the best methyl acceptor for caffeine synthase
-
-
?
S-adenosyl-L-methionine + 1,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
Q2HXL8
i.e. paraxanthine, alternative route in caffeine biosynthesis
i.e. caffeine
-
?
S-adenosyl-L-methionine + 1,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
Q2HXL8
i.e. paraxanthine, preferred substrate
i.e. caffeine
-
?
S-adenosyl-L-methionine + 1-methylxanthine
S-adenosyl-L-homocysteine + 1,3-dimethylxanthine
-
low activity
i.e. theophylline
-
?
S-adenosyl-L-methionine + 1-methylxanthine
S-adenosyl-L-homocysteine + 1,3-dimethylxanthine
low activity with the recombinant enzyme, poor activity with the native enzyme
i.e. theophylline
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
-
-
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
B9VI89
-
-
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
-
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
-
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
K9MNV5
best substrate
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
the enzyme is part of a caffeine biosynthetic pathway which includes a recycling of adenosine released from S-adenosyl-L-methionine in form of xanthosine monophosphate, overview
-
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
-
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
-
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
i.e. caffeine
-
ir
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
i.e. caffeine
-
ir
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
last step in caffeine biosynthesis pathway
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
last step in caffeine biosynthesis pathway
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
last step in caffeine biosynthesis pathway
i.e. caffeine
-
ir
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
last step in caffeine biosynthesis pathway
i.e. caffeine
-
ir
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
step of the caffeine biosynthesis, caffeine accumulation in seeds
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
-
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
-
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
-
i.e. caffeine
-
ir
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
last step in caffeine biosynthesis pathway
i.e. caffeine
-
ir
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
step of the caffeine biosynthesis, caffeine accumulation in seeds
i.e. caffeine
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
Coffea sp.
-
-
-
-
?
S-adenosyl-L-methionine + 3-methylxanthine
S-adenosyl-L-homocysteine + 1,3-dimethylxanthine
-
low activity
i.e. theophylline
-
?
S-adenosyl-L-methionine + 3-methylxanthine
S-adenosyl-L-homocysteine + 1,3-dimethylxanthine
poor activity with the recombinant enzyme, low activity with the native enzyme
i.e. theophylline
-
?
S-adenosyl-L-methionine + 7-methylxanthine
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
-
-
-
-
?
S-adenosyl-L-methionine + 7-methylxanthine
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
B9VI89
-
-
-
?
S-adenosyl-L-methionine + 7-methylxanthine
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
K9MNV5
-
i.e.theobromine
-
?
S-adenosyl-L-methionine + 7-methylxanthine
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
-
i.e. theobromine
-
?
S-adenosyl-L-methionine + 7-methylxanthine
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
Coffea sp.
-
-
-
-
?
additional information
?
-
substrate specificity, overview
-
-
?
additional information
?
-
-
the bifunctional enzyme catalyzes the last two steps of the caffeine biosynthesis
-
-
?
additional information
?
-
the bifunctional enzyme preferably catalyzes the reaction of the theobromine synthase, EC 2.1.1.159
-
-
?
additional information
?
-
-
the bifunctional enzyme preferably catalyzes the reaction of the theobromine synthase, EC 2.1.1.159
-
-
?
additional information
?
-
-
substrate specificity, overview, the bifunctional enzyme mainly catalyzes the reaction of the theobromine synthase, EC 2.1.1.159, no activity with xanthosine, xanthosine 5-phosphate, and theophylline
-
-
?
additional information
?
-
substrate specificity, overview, the bifunctional enzyme preferably catalyzes the reaction of the theobromine synthase, EC 2.1.1.159, no activity with xanthosine
-
-
?
additional information
?
-
-
substrate specificity, overview, the bifunctional enzyme preferably catalyzes the reaction of the theobromine synthase, EC 2.1.1.159, no activity with xanthosine
-
-
?
additional information
?
-
B9VI89
tea caffeine synthase is a bifunctional enzyme comprising two S-adenosyl-L-methionine-dependent N-methyltransferase activities leading to the formation of theobromine and caffeine
-
-
?
additional information
?
-
-
tea caffeine synthase is a bifunctional enzyme comprising two S-adenosyl-L-methionine-dependent N-methyltransferase activities leading to the formation of theobromine and caffeine
-
-
?
additional information
?
-
bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview
-
-
?
additional information
?
-
enzyme expression and activity during caffeine biosynthesis in fruits, overview
-
-
?
additional information
?
-
-
feeding experiments with fruit samples, determination of reaction products, overview
-
-
?
additional information
?
-
substrate specificity, bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview
-
-
?
additional information
?
-
-
substrate specificity, overview, no activity with xanthine, hypoxanthine, and xanthosine
-
-
?
additional information
?
-
the multifunctional isozyme DXMT1 catalyzes also the reaction theobromine synthase, EC 2.1.1.159, but at lower activity compared to its N1-methyltransferase, i.e. caffeine synthase activity
-
-
?
additional information
?
-
-
enzyme expression and activity during caffeine biosynthesis in fruits, overview
-
-
?
additional information
?
-
the bifunctional enzyme also catalyzes the reaction of the theobromine synthase, EC 2.1.1.159
-
-
?
additional information
?
-
the bifunctional enzyme also catalyzes the reaction of the theobromine synthase, EC 2.1.1.159
-
-
?
additional information
?
-
Coffea sp.
-
the descending order of activity of the purine base with caffeine synthase is N-3, N-1, N-7
-
-
?
