2.1.1.163: demethylmenaquinone methyltransferase
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For detailed information about demethylmenaquinone methyltransferase, go to the full flat file.
Reaction
Synonyms
2-heptaprenyl-1,4-naphthoquinone methyltransferase, DMK methyltransferase, Dmkt1, DmtH protein, menaquinone biosynthesis methyltransferase, menG, UbiE
ECTree
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Engineering
Engineering on EC 2.1.1.163 - demethylmenaquinone methyltransferase
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G142D
mutant accumulates 2-octaprenyl-6-methoxy-1,4-benzoquinone and demethylmenaquinone as predominant intermediates
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mutant defective in the demthylmenaquinone methyltransferase activity expresses the same formate-dependent nitrite reduction activity as the parental strain
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mutant ubiE lacks ubiquinone due to defect in a specific methylation step of ubiquinone synthesis. Synthesis of menaquinone from demethylmenaquinone depends on the same gene ubiE. Mutant contains only demethylmenaquinone, but not menaquinone. Strain is able to grow with fumarate, trimethylamine N-oxide and dimethylsulfoxide, but not with nitrate as electron acceptor. Anaerobic respiration with fumarate and trimethylamine are catalyzed at 69% and 74% of wild-type rates, respectively. Dimethylsulfoxide respiration is reduced to 38% of wild-type, and nitrate respiration is below 8%
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mutant ubiE lacks ubiquinone due to defect in a specific methylation step of ubiquinone synthesis. Synthesis of menaquinone from demethylmenaquinone depends on the same gene ubiE. Mutant contains only demethylmenaquinone, but not menaquinone. Strain is able to grow with fumarate, trimethylamine N-oxide and dimethylsulfoxide, but not with nitrate as electron acceptor. Anaerobic respiration with fumarate and trimethylamine are catalyzed at 69% and 74% of wild-type rates, respectively. Dimethylsulfoxide respiration is reduced to 38% of wild-type, and nitrate respiration is below 8%
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expression in Escherichia coli complements mutation AN70 deficient of a specific methyltransferase in the ubiquinone biosynthetic pathway
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expression in Escherichia coli complements mutation AN70 deficient of a specific methyltransferase in the ubiquinone biosynthetic pathway
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construction of dmtH null mutant strain HK116, symbiotic phenoytpe, HK116 forms small abnormal white nodules with a host plant, but lacks nitrogen-fixing ability. Nitrogenase activity of the nodules induced by HK116 is undetectable, and the number of nodules remarkably decreases compared to wild type 7653R
additional information
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construction of dmtH null mutant strain HK116, symbiotic phenoytpe, HK116 forms small abnormal white nodules with a host plant, but lacks nitrogen-fixing ability. Nitrogenase activity of the nodules induced by HK116 is undetectable, and the number of nodules remarkably decreases compared to wild type 7653R
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