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2.1.1.164: demethylrebeccamycin-D-glucose O-methyltransferase

This is an abbreviated version!
For detailed information about demethylrebeccamycin-D-glucose O-methyltransferase, go to the full flat file.

Reaction

4'-demethylrebeccamycin
+
S-adenosyl-L-methionine
=
rebeccamycin
 +
S-adenosyl-L-homocysteine

Synonyms

rebeccamycin MT, rebeccamycin O-methyltransferase, RebM, RebM.

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.164 demethylrebeccamycin-D-glucose O-methyltransferase

Engineering

Engineering on EC 2.1.1.164 - demethylrebeccamycin-D-glucose O-methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C70A
kcat for S-adenosyl-L-methionine is nearly identical to wild-type value
C70S
kcat for S-adenosyl-L-methionine is 1.53fold higher than wild-type value
D166A
kcat for S-adenosyl-L-methionine is 10% of wild-type value
H140A
kcat for S-adenosyl-L-methionine is 5% of wild-type value
H141A
kcat for S-adenosyl-L-methionine is 21% of wild-type value
H149A/H141A
activity is below the detection limit
L136V
kcat for S-adenosyl-L-methionine is 54% of wild-type value
P75S
although properly folded based upon CD spectroscopy, the mutant displays a substantially reduced affinity for AdoMet (about 10fold increase in Km). kcat for S-adenosyl-L-methionine is 13% of wild-type value
S138A
kcat for S-adenosyl-L-methionine is 49% of wild-type value
W134Y
kcat for S-adenosyl-L-methionine is 35% of wild-type value