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2.1.1.165: methyl halide transferase

This is an abbreviated version!
For detailed information about methyl halide transferase, go to the full flat file.

Word Map on EC 2.1.1.165

Reaction

S-adenosyl-L-methionine
+
Iodide
=
S-adenosyl-L-homocysteine
+
methyl iodide

Synonyms

AtHOL1, AtHOL2, AtHOL3, halide/bisulfide methyltransferase, HMT, HMT/HTMT, HOL, HTMT, MCT, methyl chloride transferase, methyl halide transferase, MHT, S-adenosyl-L-methionine: halide ion methyltransferase, S-adenosyl-L-methionine:halide/bisulfide methyltransferase, S-adenosylmethionine-dependent halide/thiol methyltransferase, SAM:halide ion methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.165 methyl halide transferase

Storage Stability

Storage Stability on EC 2.1.1.165 - methyl halide transferase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
- 80°C, enzyme after the first gel filtration purification step, in 25 mM Tris acetate, pH 7.4, 10% glycerol, and 14 mM 2-mercaptoethanol, stable for over 2 months
-
-20°C to 4°C, partially purified enzyme, complete loss of activity overnight, also in the presence of protease inhibitors
-
-20°C, enzyme forms an aggregate with molecular mass of approximately 500000 Da
-20°C, enzyme in cell extract is unstable and loses activities almost completely upon storage even if dithioerythritol, EDTA, protease inhibitor or glycerol are added to the extracts
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 40% of the iodide methyltransferase activity
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 60% of the iodide methyltransferase activity
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 90% of the iodide methyltransferase activity
-80°C, after affinity chromatography, the halide/bisulfide methyltransferase becomes extremely labile losing all activity after overnight storage
-
20°C, enzyme after the affinity chromatography purification step, 25 mM Tris acetate, pH 7.4, 14 mM 2-mercaptoethanol, and 30% glycerol, 12% remaining activity after 48 h
-
4°C, enzyme after anion exchange purification step, in 25 mM Tris acetate, pH 7.4, 14 mM 2-mercaptoethanol, and 175 mM NaCl, more than 70% remaining activity after 24 h and 55% after 48 h
-
4°C, enzyme in cell extract is unstable and loses activities almost completely upon storage even if dithioerythritol, EDTA, protease inhibitor or glycerol are added to the extracts