2.1.1.167: 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase
This is an abbreviated version!
For detailed information about 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.167
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2.1.1.167
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rrnas
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nucleolar
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2'-o-ribose
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pre-ribosomal
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coimmunoprecipitation
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cold-sensitive
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methyltransferases
- 2.1.1.167
- rrnas
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nucleolar
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2'-o-ribose
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pre-ribosomal
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coimmunoprecipitation
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cold-sensitive
- methyltransferases
Reaction
Synonyms
Spb1p
ECTree
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Substrates Products
Substrates Products on EC 2.1.1.167 - 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase
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REACTION DIAGRAM
S-adenosyl-L-methionine + guanosine2922 in 27S pre-rRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine2922 in 27S pre-rRNA
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Spb1p is a site-specific 2'-O-ribose RNA MTase that catalyzes the formation of Gm2922, a universally conserved position of the catalytic center of the ribosome that is essential for translation. The enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation. 2'-O-methylguanosine2922 appears at a late processing stage, during the maturation of the 27S pre-rRNA
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S-adenosyl-L-methionine + uridine2918 in 25S rRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA
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the enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation
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S-adenosyl-L-methionine + uridine2918 in 25S rRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA
the enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation
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S-adenosyl-L-methionine + uridine2918 in 25S rRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA
no methylation of uridine2791
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Spb1p is required for proper assembly of pre-ribosomal particles during the biogenesis of 60S ribosomal subunits
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additional information
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Spb1p is required for proper assembly of pre-ribosomal particles during the biogenesis of 60S ribosomal subunits
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additional information
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a point mutation in the Ado-Met binding site of Spb1p affects cell growth but does not abolish methylation of U2918. When this mutation is combined with disruption of snR52 cell growth is severely impaired and U2918 is no longer methylated. In vitro, Spb1p is able to methylate U2918 on 60S subunits. For this methylation two mechanisms coexist: a site-specific methyltransferase (Spb1p) and a snoRNA-dependent mechanism
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additional information
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a point mutation in the Ado-Met binding site of Spb1p affects cell growth but does not abolish methylation of U2918. When this mutation is combined with disruption of snR52 cell growth is severely impaired and U2918 is no longer methylated. In vitro, Spb1p is able to methylate U2918 on 60S subunits. For this methylation two mechanisms coexist: a site-specific methyltransferase (Spb1p) and a snoRNA-dependent mechanism
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