2.1.1.169: tricetin 3',4',5'-O-trimethyltransferase
This is an abbreviated version!
For detailed information about tricetin 3',4',5'-O-trimethyltransferase, go to the full flat file.
Word Map on EC 2.1.1.169
Reaction
3 S-adenosyl-L-methionine
+
Synonyms
FOMT, O-methyltransferase, OMT2, TaCOMT1, TaOMT, TaOMT2
ECTree
2.1.1.169 tricetin 3',4',5'-O-trimethyltransferaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.1.1.169 - tricetin 3',4',5'-O-trimethyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3 S-adenosyl-L-methionine + tricetin
3 S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin
S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
S-adenosyl-L-methionine + 3'-O-methyltricetin
S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
S-adenosyl-L-methionine + 5-hydroxyferulic acid
?
second most preferred substrate with 78% activity compared to tricetin
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?
S-adenosyl-L-methionine + tricetin
S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
3 S-adenosyl-L-methionine + tricetin
3 S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
overall reaction
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?
3 S-adenosyl-L-methionine + tricetin
3 S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
TaOMT1 (TaCOMT1) catalyzes the sequential methylation of tricetin to its 3'-O-monomethyl-derivative (selgin), 3',5'-O-dimethyl-derivative (tricin) and 3',4',5'-O-trimethyl derivative. 3',5'-O-dimethyltricetin appears to be the major reaction product. The enzyme also catalyzes methylation of luteolin, quercetin and eriodictyol
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?
3 S-adenosyl-L-methionine + tricetin
3 S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
the enzyme catalyzes three sequential O-methylations, forming 3'-O-methyltricetin, 3',5'-dimethyltricetin and 3',4',5'-O-trimethyltricetin. Tricetin is the preferred substrate. Also catalyzes the methylation of 5-hydroxyferulic acid, luteolin, quercetin, eriodyctiol, quercetagetin, taxifolin, gossypetin and myricetin. TaOMT2 is a flavonoid, B-ring-specific O-methyltransferase with a preference for flavones > dihydroflavones > flavonols that possess at least two, B-ring hydroxyl groups
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?
S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin
S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
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?
S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin
S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
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?
S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin
S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
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?
S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin
S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
the enzyme catalyzes three sequential O-methylations, forming 3'-O-methyltricetin, 3',5'-dimethyltricetin and 3',4',5'-O-trimethyltricetin. Tricetin is the preferred substrate
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?
S-adenosyl-L-methionine + 3'-O-methyltricetin
S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
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?
S-adenosyl-L-methionine + 3'-O-methyltricetin
S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
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?
S-adenosyl-L-methionine + 3'-O-methyltricetin
S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
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?
S-adenosyl-L-methionine + 3'-O-methyltricetin
S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
the enzyme catalyzes three sequential O-methylations, forming 3'-O-methyltricetin, 3',5'-dimethyltricetin and 3',4',5'-O-trimethyltricetin. Tricetin is the preferred substrate
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?
S-adenosyl-L-methionine + tricetin
S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
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?
S-adenosyl-L-methionine + tricetin
S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
i.e. 5,7,3',4',5'-pentahydroxyflavone, most preferred substrate
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?
S-adenosyl-L-methionine + tricetin
S-adenosyl-L-homocysteine + 3'-O-methyltricetin
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?
S-adenosyl-L-methionine + tricetin
S-adenosyl-L-homocysteine + 3'-O-methyltricetin
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?
S-adenosyl-L-methionine + tricetin
S-adenosyl-L-homocysteine + 3'-O-methyltricetin
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?
S-adenosyl-L-methionine + tricetin
S-adenosyl-L-homocysteine + 3'-O-methyltricetin
the enzyme catalyzes three sequential O-methylations, forming 3'-O-methyltricetin, 3',5'-dimethyltricetin and 3',4',5'-O-trimethyltricetin. Tricetin is the preferred substrate
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?
additional information
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stepwise methylation of tricetin involving deprotonation of its hydroxyl groups by a His262-Asp263 pair followed by nucleophilic attack of SAM-methyl groups, substrate binding is mediated by an extensive network of H-bonds and van der Waals interactions, residue Val309 determines substrate specificity for tricetin and may define the evolutionary differences between the two closely related proteins, COMT and flavone OMT, molecular modeling, overview
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?
additional information
?
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stepwise methylation of tricetin involving deprotonation of its hydroxyl groups by a His262-Asp263 pair followed by nucleophilic attack of SAM-methyl groups, substrate binding is mediated by an extensive network of H-bonds and van der Waals interactions, residue Val309 determines substrate specificity for tricetin and may define the evolutionary differences between the two closely related proteins, COMT and flavone OMT, molecular modeling, overview
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?
additional information
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structure-function relationships, homology modeling and molecular docking using MsCOMT crystal structure, PDB ID 1KYZ, overview
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additional information
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structure-function relationships, homology modeling and molecular docking using MsCOMT crystal structure, PDB ID 1KYZ, overview
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