2.1.1.173: 23S rRNA (guanine2445-N2)-methyltransferase
This is an abbreviated version!
For detailed information about 23S rRNA (guanine2445-N2)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.173
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2.1.1.173
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ether
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n-acetylmuramic
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rrnas
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plasmalogens
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6-phosphate
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coa
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murnac
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haemophilus
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renamed
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peptidoglycan
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citrate
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glucokinase
- 2.1.1.173
- ether
-
n-acetylmuramic
- rrnas
-
plasmalogens
-
6-phosphate
- coa
- murnac
-
haemophilus
-
renamed
- peptidoglycan
- citrate
- glucokinase
Reaction
Synonyms
23S RNA m(2)G2445 methyltransferase, EC 2.1.1.51, EC 2.1.1.52, m2G2445 methyltransferase, ribosomal large subunit methyltransferase, RlmKL, RlmL, Smu472, ycbY
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General Information
General Information on EC 2.1.1.173 - 23S rRNA (guanine2445-N2)-methyltransferase
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evolution
malfunction
physiological function
additional information
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RlmKL is involved in the efficient assembly of 50S subunit in a mutant strain lacking an RNA helicase deaD
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the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered
evolution
the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered
insertions in both uup and ycbY confer a Uup- phenotype. It is concluded that ycbY and uup constitute a single operon and that the failure of the ycbY insertion to complement mutations in uup is because of a polarity effect associated with the former
malfunction
knock-out of the ycbY gene leads to loss of modification at G2445 and growth retardation. Growth competition with the parental wild-type strain leads to a gradual decrease in the knock-out strain cells proportion in the media
malfunction
lack of G2445 methylation as a consequence of deletion of the rlmL(ycbY) gene causes significant growth retardation
malfunction
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deletion of rlmL/ycbY results in a slight growth reduction phenotype
it is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center
physiological function
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cooperative methylation of helix 74 by RlmKL plays a key role in the efficient assembly of the 50S subunit, RlmKL enzyme is an example of a methyltransferase catalyzing two mechanistically different types of RNA modification. RlmKL has an unwinding activity of Helix 74, facilitating cooperative methylations of m7G2069 and m2G2445 during biogenesis of 50S subunit. Methyltransferase RlmL, encoded by rlmL/ycbY, catalyzes S-adenosyl-L-methionine-dependent m2G2445 formation
physiological function
the N-terminal region of YcbY adds the m2G2445 modification, while the C-terminal region of YcbY is responsible for the m7G2069 methylation on the opposite side of the same helix, H74. YcbY enzyme is an example of a methyltransferase catalyzing two mechanistically different types of RNA modification