2.1.1.173: 23S rRNA (guanine2445-N2)-methyltransferase

This is an abbreviated version!
For detailed information about 23S rRNA (guanine2445-N2)-methyltransferase, go to the full flat file.

Reaction

Synonyms

23S RNA m(2)G2445 methyltransferase, EC 2.1.1.51, EC 2.1.1.52, m2G2445 methyltransferase, ribosomal large subunit methyltransferase, RlmKL, RlmL, Smu472, ycbY

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.173 23S rRNA (guanine2445-N2)-methyltransferase

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Results	in table
7991	AA Sequence
1	CAS Registry Number
4	Cloned(Commentary)
4	Cofactor
1	Crystallization (Commentary)
10	General Information
1	Metals/Ions
4	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
8	Organism
4	PDB ID
1	pH Optimum
2	Protein Variants
2	Purification (Commentary)
1	Reaction
8	Reference
18	Substrates and Products (Substrate)
6	Subunits
20	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Substrates Products

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Substrates Products on EC 2.1.1.173 - 23S rRNA (guanine2445-N2)-methyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

S-adenosyl-L-methionine + guanine2069 in 23S rRNA

S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA

3 entries

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

11 entries

additional information

?

-

4 entries

S-adenosyl-L-methionine + guanine2069 in 23S rRNA

S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA

Escherichia coli

-

-

720598

-

-

?

S-adenosyl-L-methionine + guanine2069 in 23S rRNA

S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA

Escherichia coli

P75864

-

720601

-

-

?

S-adenosyl-L-methionine + guanine2069 in 23S rRNA

S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA

Escherichia coli

P75864

m7G2069 modification by YcbY is stoichiometric

720601

-

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

Escherichia coli

-

-

720598

-

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

Escherichia coli

P75864

-

689247, 718495

-

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

Escherichia coli

-

-

718495

-

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

Escherichia coli

P75864

-

720601, 720987

-

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

Escherichia coli

P75864

N2-methylguanosine2445 of the 23S rRNA is located in a cluster of modified nucleotides concentrated at the peptidyl transferase center of the ribosome. It is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center

675411

-

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

Escherichia coli

P75864

recombinant YcbY protein is able to methylate 23S rRNA purified from the ycbY knock-out strain in vitro, assembled 50S subunits are not a substrate for the methylase

675411

-

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

Escherichia coli

P75864

RlmL possessing an additional RNA-binding domain acts on naked ribosomal RNA or early assembly intermediates in the cell

689247

-

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

Escherichia coli

-

duplex formation of H74 is not required for the m2G2445 formation. The 29-mer single-stranded transcript 6, which consists of residues C2422 to A2450, can form m2G2445 efficiently

720598

-

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

Escherichia coli

P75864

m2G2445 modification by YcbY is stoichiometric

720601

-

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

Streptococcus mutans

-

-

720601

-

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

Streptococcus mutans

-

Smu472 adds the m2G2445 modification

720601

-

-

?

additional information

?

-

Escherichia coli

P75864

the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY

720601

-

-

?

additional information

?

-

Escherichia coli

-

the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY

720601

-

-

?

additional information

?

-

Escherichia coli

-

helix 80 and the 12 nt ss region are critical sites necessary for m2G2445 and m7G2069 formation. Transcript 7, which lacks helix 80 and the 12 nt single-strand region, is not methylated at either position

720598

-

-

?

additional information

?

-

Streptococcus mutans

-

Smu776 does not target G2069, and this nucleotide remains unmodified in Gram-positive rRNAs

720601

-

-

?