2.1.1.175: tricin synthase

This is an abbreviated version!
For detailed information about tricin synthase, go to the full flat file.

Reaction

2 S-adenosyl-L-methionine +

Synonyms

HvOMT1, ROMT-15, ROMT-17, ROMT9, ZmOMT1

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.175 tricin synthase

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Results	in table
2	AA Sequence
4	Cloned(Commentary)
1	Cofactor
2	General Information
2	kcat/KM [mM/s]
4	KM Value [mM]
7	Metals/Ions
4	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
7	Organism
3	pH Optimum
2	pI Value
8	Protein Variants
2	Purification (Commentary)
3	Reaction
6	Reference
5	Source Tissue
2	Storage Stability
12	Substrates and Products (Substrate)
4	Subunits
5	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]

Substrates Products

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Substrates Products on EC 2.1.1.175 - tricin synthase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

2 S-adenosyl-L-methionine + tricetin

2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin

4 entries

S-adenosyl-L-methionine + 3'-O-methyltricetin

S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin

3 entries

S-adenosyl-L-methionine + selgin

S-adenosyl-L-homocysteine + tricin

Oryza sativa Japonica Group

-

-

734972

-

-

?

S-adenosyl-L-methionine + tricetin

S-adenosyl-L-homocysteine + 3'-O-methyltricetin

3 entries

S-adenosyl-L-methionine + tricetin

S-adenosyl-L-homocysteine + selgin

Oryza sativa Japonica Group

-

-

734972

-

-

?

2 S-adenosyl-L-methionine + tricetin

2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin

Hordeum vulgare

-

stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). The enzyme also catalyzes the 3'-O-methylation of luteolin (EC 2.1.1.42) and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin

706086

-

-

?

2 S-adenosyl-L-methionine + tricetin

2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin

Oryza sativa

Q9XGP7

the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives. The enzyme also catalyzes the methylation of luteolin, myricetin (formation of the 3',5'-dimethyl ether of myricetin, EC 2.1.1.149) and caffeoyl-CoA. ROMT-15 exhibits similar Kcat/Km values for the four substrates. ROMT-15 can not utilize naringenin, apigenin, or kaempferol. The 2,3-double bond and the O-dihydroxyl group are both required for catalytic activity

700855

-

-

?

2 S-adenosyl-L-methionine + tricetin

2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin

Oryza sativa

Q9XGP7

the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives. The enzyme also catalyzes the methylation of luteolin, myricetin (formation of the 3',5'-dimethyl ether of myricetin, EC 2.1.1.149) and caffeoyl-CoA. ROMT-17 prefers tricetin. ROMT-15 can not utilize naringenin, apigenin, or kaempferol. The 2,3-double bond and the O-dihydroxyl group are both required for catalytic activity

700855

-

-

?

2 S-adenosyl-L-methionine + tricetin

2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin

Zea mays

-

the enzyme also catalyzes the 3'-O-methylation of luteolin (EC 2.1.1.42) and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin

706086

-

-

?

S-adenosyl-L-methionine + 3'-O-methyltricetin

S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin

Hordeum vulgare

-

the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives

706086

-

-

?

S-adenosyl-L-methionine + 3'-O-methyltricetin

S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin

Oryza sativa

Q9XGP7

the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives

700855

-

-

?

S-adenosyl-L-methionine + 3'-O-methyltricetin

S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin

Zea mays

-

the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives

706086

-

-

?

S-adenosyl-L-methionine + tricetin

S-adenosyl-L-homocysteine + 3'-O-methyltricetin

Hordeum vulgare

-

the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives

706086

-

-

?

S-adenosyl-L-methionine + tricetin

S-adenosyl-L-homocysteine + 3'-O-methyltricetin

Oryza sativa

Q9XGP7

the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives

700855

-

-

?

S-adenosyl-L-methionine + tricetin

S-adenosyl-L-homocysteine + 3'-O-methyltricetin

Zea mays

-

the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives

706086

-

-

?