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2.1.1.179: 16S rRNA (guanine1405-N7)-methyltransferase

This is an abbreviated version!
For detailed information about 16S rRNA (guanine1405-N7)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.179

Reaction

S-adenosyl-L-methionine
+
guanine1405 in 16S rRNA
=
S-adenosyl-L-homocysteine
 +
N7-methylguanine1405 in 16S rRNA

Synonyms

16S rRNA (m7G1405) methyltransferase, 16S rRNA methyltransferase, 16S rRNA N7 G1405 methyltransferase, 16S-RMTase, ArmA, FmrO, G1405 methyltransferase ArmA, GrmA, GrmB, Kmr, Krm, M7G1405 MTase, methyltransferase RmtC, methyltransferase Sgm, N7-G1405 16S-RMTase, NbrB, RmtA, RmtB, RmtC, RmtD, RmtD2, RmtF, RmtG, sgm, Sgm methyltransferase, Sgm MTase, sisomicin-gentamicin methylase, sisomicin-gentamicin methyltransferase, sisomicin-gentamicin resistance methylase, Smr1

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.179 16S rRNA (guanine1405-N7)-methyltransferase

Cloned

Cloned on EC 2.1.1.179 - 16S rRNA (guanine1405-N7)-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in Streptomyces lividans. The sgm gene is expressed in Micromonospora melanosporea, where its own promoter is active, and also in Escherichia coli under the control of the lacZ promoter
cloning of the sgm gene into pET-25b (+) vector with the addition of N-terminal non-cleavable His6 tag, as well as alanine mutagenesis of residues D156, D182 and R108. The constructs are co-transformed along with pGroESL into the strain BL21 (DE3) of Escherichia coli for protein expression
expressed in Escherichia coli BL21(DE3) cells
-
expression in Escherichia coli
expression in Escherichia coli, Bacillus subtilis or Staphylococcus aureus
gene fmrO, phylogenetic analysis, sequence comparisons
gene grmA, phylogenetic analysis, sequence comparisons
gene grmB, phylogenetic analysis, sequence comparisons
gene kmr, phylogenetic analysis, sequence comparisons
gene nbrB, phylogenetic analysis, sequence comparisons
gene rmtA, phylogenetic analysis, sequence comparisons
gene rmtB, phylogenetic analysis, sequence comparisons
gene rmtC, expression of wild-type enzyme in Escherichi coli Top10 cells
-
gene rmtC, phylogenetic analysis, sequence comparisons
gene rmtD2, phylogenetic analysis, sequence comparisons
gene rmtF encoded on a non-self-transferable plasmid pIP849, cotranscribed with the upstream aac(6')-Ib gene, DNA and amino acid sequence determination, analysis, and comparisons, overview. Genetic structure and phylogenetic tree
gene sgm, phylogenetic analysis, sequence comparisons
gene smr1, phylogenetic analysis, sequence comparisons
introduction of a recombinant version of the Sgm methyltransferase gene from Micromonospora zionensis into an Escherichia coli strain that has a full complement of housekeeping methyltransferases. Analyses of the 16S rRNA shows that the m5C1407-specific housekeeping methyltransferase RsmF (YebU) is outcompeted by Sgm as the resistance methyltransferase gains access to its own m7G1405 target on the 30S ribosomal subunit. A single amino acid change in Sgm, which lowers the level of conferred resistance, reduces the ability of Sgm to interfere with RsmF methylation on the 30S subunit
phylogenetic analysis, sequence comparisons
sgm is cloned into a yeast expression vector to test whether the prokaryotic methylases can modify the 18S rRNA A-site and thus confer resistance to the aminoglycoside antibiotic G-418. Sgm does not provide resistant phenotypes to yeast cells. Despite all similarities in the antibiotic binding site, methylation by Sgm does not occur in yeast, suggesting that the recognition site for these methylases could be different in 30S and 40S subunits