2.1.1.184: 23S rRNA (adenine2085-N6)-dimethyltransferase

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For detailed information about 23S rRNA (adenine2085-N6)-dimethyltransferase, go to the full flat file.

Reaction

2 S-adenosyl-L-methionine +

Synonyms

23S ribosomal RNA adenine N-6 methyltransferase, EC 2.1.1.48, ErmC, ermC 23 S rRNA methyltransferase, ErmC 23S rRNA methyltransferase, ermC methylase, ErmC methyltransferase, ErmC', ErmC' methyltransferase, ErmC' MTase, erythromycin resistance protein, rRNA methyltransferase ErmC', rRNA:m6A methyltransferase ErmC'

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.184 23S rRNA (adenine2085-N6)-dimethyltransferase

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Results	in table
322	AA Sequence
3	Application
5	Cloned(Commentary)
3	Crystallization (Commentary)
2	Expression
3	General Information
3	IC50 Value
8	Inhibitors
48	kcat/KM [mM/s]
6	Ki Value [mM]
58	KM Value [mM]
1	Localization
6	Natural Substrates/ Products (Substrates)
17	Organism
1	PDB ID
6	pH Optimum
1	pH Range
35	Protein Variants
4	Purification (Commentary)
5	Reaction
15	Reference
24	Substrates and Products (Substrate)
20	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
49	Turnover Number [1/s]

Cloned

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Cloned on EC 2.1.1.184 - 23S rRNA (adenine2085-N6)-dimethyltransferase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli BW25113B cells fused to green fluorescent protein

Escherichia coli

-

734477

expressed to a high level in Escherichia coli

Bacillus subtilis

P13956

704252

expression in Escherichia coli

Bacillus subtilis

P13956

662611, 705957

the expression of ErmC' by translational coupling to kdsB, under the control of the T7lac promoter. The pTERM31 plasmid is transformed into Escherichia coli strain BL219(DE3)/pLysS

Bacillus subtilis

P13956

485441