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2.1.1.192: 23S rRNA (adenine2503-C2)-methyltransferase

This is an abbreviated version!
For detailed information about 23S rRNA (adenine2503-C2)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.192

Reaction

2 S-adenosyl-L-methionine +

adenine2503 in 23S rRNA
+ 2 reduced [2Fe-2S] ferredoxin =
S-adenosyl-L-homocysteine
 +
L-methionine
 +
5'-deoxyadenosine
 +
2-methyladenine2503 in 23S rRNA
 + 2 oxidized [2Fe-2S] ferredoxin

Synonyms

Cfr, EC 2.1.1.194, methyltransferase YfgB/RlmN, NlmA, radical AdoMet rRNA methyltransferase, radical S-adenosyl-L-methionine enzyme, radical SAM enzyme, RlmN, RlmN methyltransferase, YfgB, YfgB/RlmN

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.192 23S rRNA (adenine2503-C2)-methyltransferase

Crystallization

Crystallization on EC 2.1.1.192 - 23S rRNA (adenine2503-C2)-methyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
C118A enzyme mutant complexed with tRNAGlu substrate, X-ray diffraction structure determination and analysis at 2.4 A resolution, structure modelling
homology modeling of structure and comparison with Cfr from Staphylococcus sciuri, EC 2.1.1.194
mutant C118A, hanging drop vapor diffusion method, using 7.5% (w/v) PEG 6000, 0.1 M HEPES, pH 7.5, and 5% (v/v) 2-methyl-2,4-pentanediol as the precipitant
-
structure of a key intermediate in the RlmN reaction, in which a Cys118Ala variant of the protein is crosslinked to a tRNAGlu substrate through the terminal methylene carbon of a formerly methylcysteinyl residue and C2 of A37. RlmN contacts the entire length of tRNAGlu, accessing A37 using an induced-fit strategy that completely unfolds the tRNA anticodon stem loop