2.1.1.199: 16S rRNA (cytosine1402-N4)-methyltransferase

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For detailed information about 16S rRNA (cytosine1402-N4)-methyltransferase, go to the full flat file.

Reaction

Synonyms

methyltransferase RsmH, mraW, RsmH

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.199 16S rRNA (cytosine1402-N4)-methyltransferase

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Results	in table
41504	AA Sequence
2	Cloned(Commentary)
1	Cofactor
1	Crystallization (Commentary)
6	General Information
6	Natural Substrates/ Products (Substrates)
38	Organism
1	PDB ID
2	Purification (Commentary)
1	Reaction
5	Reference
6	Substrates and Products (Substrate)
1	Subunits
8	Synonyms
1	Systematic Name

Crystallization

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Crystallization on EC 2.1.1.199 - 16S rRNA (cytosine1402-N4)-methyltransferase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant His6-tagged RsmH in complex with S-adenosyl-L-methionine and cytidine in a 1:5:5 ratio, sitting drop vapour diffusion method, from 20 mM Tris, 100 mM NaCl, 5% v/v glycerol, 2 mM DTT, pH 8.0, room temperature, the best crystal is obtained in the mixture solution 1.0 M ammonium sulfate, 0.1 M HEPES, pH 6.5, and 0.5% w/v PEG 8, 000 in 3-4 days, X-ray diffraction structure determination and analysis at 2.25 A resolution

Escherichia coli

P60390

720342