2.1.1.200: tRNA (cytidine32/uridine32-2'-O)-methyltransferase

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For detailed information about tRNA (cytidine32/uridine32-2'-O)-methyltransferase, go to the full flat file.

Reaction

Synonyms

2'-O-tRNA methyltransferase, PA14 14690, Saci_0621, TrMet(Xm32), TrmJ, tRNA (cytidine(32)/uridine(32)/adenosine(32)-2'-O)-methyltransferase, tRNA:Cm32/Um32 methyltransferase, tRNA:Cm32/Um32/Am32 methyltransferase, YfhQ

ECTree

2 Transferases

2.1 Transferring one-carbon groups

2.1.1 Methyltransferases

2.1.1.200 tRNA (cytidine32/uridine32-2'-O)-methyltransferase

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Results	in table
14870	AA Sequence
3	Cloned(Commentary)
2	Cofactor
3	Crystallization (Commentary)
14	General Information
1	Inhibitors
6	KM Value [mM]
2	Metals/Ions
3	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
7	Organism
21	PDB ID
2	pH Optimum
11	Protein Variants
3	Purification (Commentary)
2	Reaction
7	Reference
33	Substrates and Products (Substrate)
8	Subunits
19	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
6	Turnover Number [1/s]

Purification

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Purification on EC 2.1.1.200 - tRNA (cytidine32/uridine32-2'-O)-methyltransferase

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Escherichia coli

P0AE01

707893

recombinant C-terminally His6-tagged wild-type enzyme and deletion mutants from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration

Escherichia coli

P0AE01

736907

recombinant His-tagged complete TrmJ protein spanning residues 1-257 (TrmJ) and His-tagged N-terminal SAM-binding domain, residues 1-167, from Escherichia coli strain BL21(DE3) Rosetta T1R by nickel affinity chromatography and two different steps of gel filtration

Pseudomonas aeruginosa

A0A0H2ZF87

757835