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2.1.1.202: multisite-specific tRNA:(cytosine-C5)-methyltransferase

This is an abbreviated version!
For detailed information about multisite-specific tRNA:(cytosine-C5)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.202

Reaction

S-adenosyl-L-methionine
+
cytosine34 in tRNA precursor
=
S-adenosyl-L-homocysteine
 +
5-methylcytosine34 in tRNA precursor

Synonyms

5-methylcytosine RNA methyltransferase, broad-spectrum methyltransferase, cytosine-5 RNA methyltransferase, EC 2.1.1.29, HNSun6, M5C RNA methyltransferase, MJ0026, multisite-specific tRNA:m5C-methyltransferase, Ncl1p, NOP2/Sun RNA methyltransferase 2, NSUN2, NSUN6, PhNSUN6, RNA (cytosine-5)-methyltransferase, RNA:m5C methyltransferase, RNA:m5C MTase, TRDMT1, TRM4, TRM4A, TRM4B, Trm4p, tRNA (cytosine72-C5)-methyltransferase, tRNA-specific methyltransferase 4B, tRNA:m5C-methyltransferase, tRNA:m5C72 MTase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.202 multisite-specific tRNA:(cytosine-C5)-methyltransferase

Engineering

Engineering on EC 2.1.1.202 - multisite-specific tRNA:(cytosine-C5)-methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C271A
-
site-directed mutagenesis, the mutant enzyme forms an irreversible covalent crosslink between itself and the methylated cytosine in its target RNA
C326A
site-directed mutagenesis, tRNA binding structure compared to wild-type enzyme, the C326A mutation hampers the breakdown of the covalent intermediates
D266A
site-directed mutagenesis, the mutant shows highly increased binding affinity for SAM and highly reduced methylation activity
D293A
site-directed mutagenesis, the mutation leads to a loss of SAM binding capability and a loss of methylation activity to tRNACys
D323A
site-directed mutagenesis, the mutant shows highly increased binding affinity for SAM and highly reduced methylation activity
F141A
site-directed mutagenesis, the mutant shows activity similar to wild-type enzyme
G679R
K159A
site-directed mutagenesis, the mutant shows moderately decreased activity compared to wild-type enzyme
K159A/R181A
site-directed mutagenesis, the mutant shows highly decreased activity compared to wild-type enzyme
K160A
site-directed mutagenesis, the mutant shows moderately decreased activity compared to wild-type enzyme
K160A/R181A
site-directed mutagenesis, the mutant shows highly decreased activity compared to wild-type enzyme
K248A
site-directed mutagenesis, the mutant shows 5fold increased binding affinity for SAM
K271A
site-directed mutagenesis, the mutant shows highly increased binding affinity for SAM and highly reduced methylation activity
R181A
site-directed mutagenesis, the mutant shows highly decreased activity compared to wild-type enzyme
C260A
-
the mutant enzyme forms stable RNA-protein complexes in vitro. The complexes are formed due to faulty product release by the modified Trm4p enzyme and contain only natural ribonucleotides within the RNA
C260S
-
the mutant enzyme forms stable RNA-protein complexes in vitro. The complexes are formed due to faulty product release by the modified Trm4p enzyme and contain only natural ribonucleotides within the RNA
C310A
-
the mutant shows only a slightly lower affinity for RNA compared to wild-type Trm4p. The C310A mutant of Trm4p is unable to bind RNA covalently as well as to transfer the methyl group from SAM to the RNA substrate since no activity was detectable
C424A
-
organism containing the C424A mutation is not viable
C478A
-
organism containing the C478A mutation is viable
additional information