2.1.1.206: tRNA (cytidine56-2'-O)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (cytidine56-2'-O)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.206
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2.1.1.206
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methyltransferases
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spout
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9-like
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crenarchaeon
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2'-o-methylation
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n-methyltransferase
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pyrobaculum
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pyrococcus
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n2-guanine
- 2.1.1.206
- methyltransferases
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spout
-
9-like
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crenarchaeon
-
2'-o-methylation
- n-methyltransferase
- pyrobaculum
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pyrococcus
-
n2-guanine
Reaction
Synonyms
aTrm56, PAB1040, Ta0931, transfer RNA methyltransferase, Trm56, tRNA ribose 2'-O-methyltransferase aTrm56
ECTree
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Substrates Products
Substrates Products on EC 2.1.1.206 - tRNA (cytidine56-2'-O)-methyltransferase
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REACTION DIAGRAM
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
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-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
-
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position
-
-
?
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
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-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G
-
-
?
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
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-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
in vitro, the tRNA ribose 2'-O-methyltransferase aTrm56 specifically catalyses the AdoMet-dependent 2'-O-ribose methylation of cytidine position 56 in tRNA transcripts. In vitro transcribed Escherichia coli tRNALeu2, yeast tRNAPhe, and Pyrococcus abyssi tRNALeu(CAa) tRNAs are methylated with significant initial rates. Mutations of cytosine at position 56 either by uridine or guanine in tRNA abolish methyl incorporation. A tRNA minisubstrate corresponding to the yeast tRNAAsp transcript missing the D stem-loop, is methylated in vitro at a slightly lower efficiency than the wild-type tRNAAsp. A minisubstrate consisting of the T-loop of tRNAAsp closed by a synthetic stem is also tested. This TSL minisubstrate is still methylated in vitro even though the reaction is 4-5fold less efficient than with the wild type substrate. The complete 3D architecture of the tRNA substrate is not required for aTrm56 catalysis. A T stem-loop structure alone is thus a minimal substrate recognized by aTrm56
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?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
Pyrococcus horikoshii tRNAVal. aTrm56 prefers the L-shaped tRNA to the lambda form as its substrate
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-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
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-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
-
-
?
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
-
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
the methylated nucleotide is identified as pCm
-
-
?
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
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additional information
?
-
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-
additional information
?
-
purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed
-
-
-