2.1.1.213: tRNA (guanine10-N2)-dimethyltransferase
This is an abbreviated version!
For detailed information about tRNA (guanine10-N2)-dimethyltransferase, go to the full flat file.
Word Map on EC 2.1.1.213
-
2.1.1.213
-
carotenoid
-
dioxygenase
-
thiouridine
-
acyclic
-
thump
- 2.1.1.213
-
carotenoid
-
dioxygenase
-
thiouridine
-
acyclic
-
thump
Reaction
2 S-adenosyl-L-methionine + = 2 S-adenosyl-L-homocysteine +
Synonyms
EC 2.1.1.32, N(2),N(2)-dimethylguanosine tRNA methyltransferase, PAB1283, PabTrm-G10, PabTrm-m2 2G10 enzyme, Trm-G10, tRNA:m2(2)G10 methyltransferase
ECTree
Advanced search results
Substrates Products
Substrates Products on EC 2.1.1.213 - tRNA (guanine10-N2)-dimethyltransferase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
REACTION DIAGRAM
2 S-adenosyl-L-methionine + guanine10 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA
S-adenosyl-L-methionine + guanine10 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
additional information
?
-
-
model for a potential binding mode for the THUMP domain, which may be common to various RNA modification enzymes that specifically modify nucleotides in the 3D-core of the tRNA molecule
-
-
?
2 S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA
depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures
-
-
?
2 S-adenosyl-L-methionine + guanine10 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA
-
identity elements required for the efficient formation of N2-dimethylguanine10: uracil25 and a V-loop of four nucleotides are major determinants for the formation of N2-dimethylguanine10, whereas the identity of the 13-22 base-pair clearly influences the rate of N2-dimethylguanine10 formation. The integrity of the T-arm prolonged with the amino acid acceptor stem is an important structural element for the dimethylation step catalysed by PabTrm-m2 2G10 enzyme
-
-
?
S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
-
-
-
?
S-adenosyl-L-methionine + guanine10 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures
-
-
?