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2.1.1.214: tRNA (guanine10-N2)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (guanine10-N2)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.214

Reaction

S-adenosyl-L-methionine
+
guanine10 in tRNA
=
S-adenosyl-L-homocysteine
+
N2-methylguanine10 in tRNA

Synonyms

(m2G10) methyltransferase, archaeal Trm11, EC 2.1.1.32, eukaryotic m2G10 tRNA methyltransferase, eukaryotic Trm11, N2, N2-dimethylguanosine tRNA methyltransferase, N2,N2-dimethylguanosine tRNA methyltransferase, Tk0981, Trm-G10, Trm-m22G10, Trm11, Trm11-Trm112 complex, Trm11p, Trmp112p, tRNA m2G10/m22G10 methyltransferase, tRNA MTase, YOL124c

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.214 tRNA (guanine10-N2)-methyltransferase

Purification

Purification on EC 2.1.1.214 - tRNA (guanine10-N2)-methyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged Trm112p, alone or with Trm9p, from Escherichia coli strain S15 (DE3-pLysS) by nickel affinity chromatography and desalting gel filtration
-
recombinant soluble C-terminally His6-tagged wild-type and mutant enzymes to homogeneity from Escherichia coli strain BL21-Gold (DE3) by nickel affinity chromatography and gel filtration