2.1.1.221: tRNA (guanine9-N1)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (guanine9-N1)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.221
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2.1.1.221
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microcephaly
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archaea
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disability
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intellectual
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purine
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stature
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eukarya
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n1-methylation
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monogenic
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puberty
- 2.1.1.221
- microcephaly
- archaea
- disability
-
intellectual
- purine
-
stature
- eukarya
-
n1-methylation
-
monogenic
- puberty
Reaction
Synonyms
EC 2.1.1.31, hTRMT10A, m1G9 methyltransferase, m1G9 MTase, m1R9-specific TkTrm10, More, ScTrm10, SPOUT MTase, TK0422p, TkTrm10, Trm10, Trm10p (ambiguous), TRMT10A, Trmt10C, tRNA (guanine-N(1)-)-methyltransferase, tRNA m1G9 methyltransferase, tRNA m1G9 MTase, tRNA m1G9 SPOUT methyltransferase, tRNA m1R9 methyltransferase, tRNA(m1G9/m1A9)-methyltransferase, tRNA(m1G9/m1A9)MTase
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Metals Ions
Metals Ions on EC 2.1.1.221 - tRNA (guanine9-N1)-methyltransferase
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Mg2+
additional information
Mg2+
required, the two tRNAs from Saccharomyces cerevisiae requires a much higher Mg2+ concentration (6-10 mM in the assay) for maximal TkTrm10 activity compared to the two Thermococcus kodakarensis tRNAs, for which maximal activity is observed at about 1 mM Mg2+ or less. Similar trends are exhibited for both m1G9 and m1A9 reactions, indicating that the identity of the target purine does not affect the observed metal dependencies
additional information
Trm10 does not depend on a catalytic metal ion
additional information
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Trm10 does not depend on a catalytic metal ion
additional information
no activity is detected in the absence of metal
additional information
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no activity is detected in the absence of metal